Abstract
Genes for three enzymes of intermediary sugar metabolism in E. coli, zwf (glucose 6-phosphate dehydrogenase, constitutive), edd (gluconate 6-phosphate dehydrase, inducible), and eda (2-keto-3-deoxygluconate 6-phosphate aldolase, differently inducible) are closely linked on the E. coli genetic map, the overall gene order being man... old... eda. edd. zwf... cheB... uvrC... his. One class of apparent revertants of an eda mutant strain contains a secondary mutation in edd, and some of these mutations are deletions extending into zwf. We have used a series of spontaneous edd-zwf deletions to map a series of point mutants in zwf and thus report the first fine structure map of a gene for a constitutive enzyme (zwf).
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Selected References
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- Armstrong J. B., Adler J. Location of genes for motility and chemotaxis on the Escherichia coli genetic map. J Bacteriol. 1969 Jan;97(1):156–161. doi: 10.1128/jb.97.1.156-161.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curtiss R., 3rd, Caro L. G., Allison D. P., Stallions D. R. Early stages of conjugation in Escherichia coli. J Bacteriol. 1969 Nov;100(2):1091–1104. doi: 10.1128/jb.100.2.1091-1104.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Faik P., Kornberg H. L., McEvoy-Bowe E. Isolation and properties of Escherichia coli mutants defective in 2-keto 3-deoxy 6-phosphogluconate aldolase activity. FEBS Lett. 1971 Dec 15;19(3):225–228. doi: 10.1016/0014-5793(71)80519-7. [DOI] [PubMed] [Google Scholar]
- Fradkin J. E., Fraenkel D. G. 2-keto-3-deoxygluconate 6-phosphate aldolase mutants of Escherichia coli. J Bacteriol. 1971 Dec;108(3):1277–1283. doi: 10.1128/jb.108.3.1277-1283.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zdarek J., Fraenkel G. Neurosecretory control of ecdysone release during puparium formation of flies. Gen Comp Endocrinol. 1971 Dec;17(3):483–489. doi: 10.1016/0016-6480(71)90183-3. [DOI] [PubMed] [Google Scholar]