Skip to main content
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1975 Feb;53(2):229–234. doi: 10.1111/j.1476-5381.1975.tb07353.x

Synthesis of kallikreins by rat kidney slices.

K Nustad, K Vaaje, J V Pierce
PMCID: PMC1666284  PMID: 1148483

Abstract

1 Four radioactive akllikreins were isolated from rat kidney slices incubated with (3H)-L-leucine. 2 The kallikreins were purified by procedures previously used for the isolation of rat urinary kallikreins (Nustad & Pierce, 1974), and by affinity chromatography on a column of insolubilized anti-rat urinary kallikrein. 3 The kidney kallikreins resembled the urinary kallikreins in their relative amounts, isoelectric points and electrophoretic mobilities on polyacrylamide disc gels. 4 The data indicate that the kidney synthesizes four kallikreins which are released into urine. The kallikreins are not changed by passage through the lower urinary tract.

Full text

PDF
229

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adetuyibi A., Mills I. H. Relation between urinary kallikrein and renal function, hypertension, and excretion of sodium and water in man. Lancet. 1972 Jul 29;2(7770):203–207. doi: 10.1016/s0140-6736(72)91636-4. [DOI] [PubMed] [Google Scholar]
  2. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  3. Carvalho I. F., Diniz C. R. Kinin-forming enzyme (kininogenin) in homogenates of rat kidney. Biochim Biophys Acta. 1966 Oct 17;128(1):136–148. doi: 10.1016/0926-6593(66)90150-0. [DOI] [PubMed] [Google Scholar]
  4. Chernova N. A., Nekrasova A. A. Kallikrein mochi pri gipertonicheskoi bolezni i renovaskuliarnoi gipertonii. Kardiologiia. 1971;11(4):84–89. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Margolis H. S., Geller R., Pisano J. J., Sjoerdsma A. Altered urinary kallikrein excretion in human hypertension. Lancet. 1971 Nov 13;2(7733):1063–1065. doi: 10.1016/s0140-6736(71)90382-5. [DOI] [PubMed] [Google Scholar]
  7. Margolius H. S., Geller R. G., De Jong W., Pisano J. J., Sjoerdsma A. Urinary kallikrein excretion in hypertension. Circ Res. 1972 Sep;31(9 Suppl):125–131. [PubMed] [Google Scholar]
  8. Marin-Grez M., Cottone P., Carretero O. A. Evidence for an involvement of kinins in regulation of sodium excretion. Am J Physiol. 1972 Oct;223(4):794–796. doi: 10.1152/ajplegacy.1972.223.4.794. [DOI] [PubMed] [Google Scholar]
  9. McGiff J. C., Crowshaw K., Itskovitz H. D. Prostaglandins and renal function. Fed Proc. 1974 Jan;33(1):39–47. [PubMed] [Google Scholar]
  10. McGiff J. C., Terragno N. A., Malik K. U., Lonigro A. J. Release of a prostaglandin E-like substance from canine kidney by bradykinin. Circ Res. 1972 Jul;31(1):36–43. doi: 10.1161/01.res.31.1.36. [DOI] [PubMed] [Google Scholar]
  11. Nustad K., Pierce J. V. Purification of rat urinary kallikreins and their specific antibody. Biochemistry. 1974 May 21;13(11):2312–2319. doi: 10.1021/bi00708a012. [DOI] [PubMed] [Google Scholar]
  12. Nustad K. The relationship between kidney and urinary kininogenase. Br J Pharmacol. 1970 May;39(1):73–86. doi: 10.1111/j.1476-5381.1970.tb09557.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ROBERTS P. S. Measurement of the rate of plasmin action on synthetic substrates. J Biol Chem. 1958 May;232(1):285–291. [PubMed] [Google Scholar]
  14. ROBERTS P. S. The esterase activities of human plasmin during purification and subsequent activation by streptokinase or glycerol. J Biol Chem. 1960 Aug;235:2262–2266. [PubMed] [Google Scholar]
  15. Sardesai V. M. Determination of bradykinin in blood and bradykininogen in tissues. Can J Physiol Pharmacol. 1968 Jan;46(1):77–79. doi: 10.1139/y68-013. [DOI] [PubMed] [Google Scholar]
  16. Stoner J., Manganiello V. C., Vaughan M. Effects of bradykinin and indomethacin on cyclic GMP and cyclic AMP in lung slices. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3830–3833. doi: 10.1073/pnas.70.12.3830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Werle E., Zach P. Verteilung von Kininogen in Serum und Geweben bei Ratten und anderen Säugetieren. Z Klin Chem Klin Biochem. 1970 May;8(3):186–189. [PubMed] [Google Scholar]
  18. Young R. W., Fulhorst H. W. Recovery of S35 radioactivity from protein-bearing polyacrylamide gel. Anal Biochem. 1965 May;11(2):389–391. doi: 10.1016/0003-2697(65)90030-8. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES