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. 1973 Jan 1;56(1):191–205. doi: 10.1083/jcb.56.1.191

AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES

M R Adelman 1, Gunter Blobel 1, David D Sabatini 1
PMCID: PMC2108827  PMID: 4345164

Abstract

A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.

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Selected References

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