Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Mar;149(3):1166–1170. doi: 10.1128/jb.149.3.1166-1170.1982

Stimulation of groE synthesis in Escherichia coli by bacteriophage lambda infection.

J Kochan, H Murialdo
PMCID: PMC216514  PMID: 6460752

Abstract

We found that infection of Escherichia cell by lambda results in at least a twofold stimulation in the rate of synthesis of one of the products of groE. To determine what lambda-coded factors were responsible for this stimulation, numerous phage lambda mutants carrying bio substitutions were analyzed for their ability to stimulate groE synthesis. Our results revealed that the main factor(s) which is responsible for stimulating groE synthesis is located between the endpoints of the lambda bio69 and lambda bio252 substitutions, a region of DNA coding for bet, gam, kil, and cIII.

Full text

PDF
1166

Images in this article

1167Image
on p.1167
1168Image
on p.1168

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloch P. L., Phillips T. A., Neidhardt F. C. Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins. J Bacteriol. 1980 Mar;141(3):1409–1420. doi: 10.1128/jb.141.3.1409-1420.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen S. N., Chang A. C. Genetic expression in bacteriophage lambda. 3. Inhibition of Escherichia coli nucleic acid and protein synthesis during lambda development. J Mol Biol. 1970 May 14;49(3):557–575. doi: 10.1016/0022-2836(70)90281-0. [DOI] [PubMed] [Google Scholar]
  3. Court D., Gottesman M., Gallo M. Bacteriophage lambda hin function. I. Pleiotropic alteration in host physiology. J Mol Biol. 1980 Apr 25;138(4):715–729. doi: 10.1016/0022-2836(80)90061-3. [DOI] [PubMed] [Google Scholar]
  4. Georgiou M., Georgopoulos C. P., Eisen H. An analysis of the Tro phenotype of bacteriophage lambda. Virology. 1979 Apr 15;94(1):38–54. doi: 10.1016/0042-6822(79)90436-7. [DOI] [PubMed] [Google Scholar]
  5. Georgopoulos C. P., Hendrix R. W., Casjens S. R., Kaiser A. D. Host participation in bacteriophage lambda head assembly. J Mol Biol. 1973 May 5;76(1):45–60. doi: 10.1016/0022-2836(73)90080-6. [DOI] [PubMed] [Google Scholar]
  6. Greer H. The kil gene of bacteriophage lambda. Virology. 1975 Aug;66(2):589–604. doi: 10.1016/0042-6822(75)90231-7. [DOI] [PubMed] [Google Scholar]
  7. Hohn T., Hohn B., Engel A., Wurtz M., Smith P. R. Isolation and characterization of the host protein groE involved in bacteriophage lambda assembly. J Mol Biol. 1979 Apr 15;129(3):359–373. doi: 10.1016/0022-2836(79)90501-1. [DOI] [PubMed] [Google Scholar]
  8. Hohn T., Hohn B., Engel A., Wurtz M., Smith P. R. Isolation and characterization of the host protein groE involved in bacteriophage lambda assembly. J Mol Biol. 1979 Apr 15;129(3):359–373. doi: 10.1016/0022-2836(79)90501-1. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Murialdo H. Early intermediates in bacteriophage lambda prohead assembly. Virology. 1979 Jul 30;96(2):341–367. doi: 10.1016/0042-6822(79)90094-1. [DOI] [PubMed] [Google Scholar]
  11. Murialdo H., Siminovitch L. The morphogenesis of bacteriophage lambda. IV. Identification of gene products and control of the expression of the morphogenetic information. Virology. 1972 Jun;48(3):785–823. doi: 10.1016/0042-6822(72)90162-6. [DOI] [PubMed] [Google Scholar]
  12. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  13. Sternberg N. Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). II. The propagation of phage lambda. J Mol Biol. 1973 May 5;76(1):25–44. doi: 10.1016/0022-2836(73)90079-x. [DOI] [PubMed] [Google Scholar]
  14. Szybalski E. H., Szybalski W. A comprehensive molecular map of bacteriophage lambda. Gene. 1979 Nov;7(3-4):217–270. doi: 10.1016/0378-1119(79)90047-7. [DOI] [PubMed] [Google Scholar]
  15. Tilly K., Murialdo H., Georgopoulos C. Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1629–1633. doi: 10.1073/pnas.78.3.1629. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES