Abstract
The mouse T cell alloantigen, Tsud, is expressed on a minority of mature, Lyt-2+ cells, and its expression is controlled by a gene linked to the immunoglobulin heavy chain gene cluster, Igh. Tsud can be assayed by immunofluorescence staining with an antiserum made in BALB/c mice against C.AL-20 concanavalin A blasts. This antiserum can also be used to induced T suppressor cells in mice expressing Tsu(d). Both of these assays were used to type several panels of recombinant inbred strains and Igh recombinant strains to accurately map the Tsu(d) locus. The Tsu(d) gene is located very near the heavy chain constant-region genes, Igh-C, on the side toward the prealbumin gene, Pre-1. Tsu(d) is not among the heavy chain variable-region genes, Igh-V, and thus is not a variable-region framework allotype, subgroup determinant, or idiotype. The map position suggest that the Tsu(d) antigen is a constant region allotypic determinant on the as yet uncharacterized T cell receptor.
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Selected References
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- Coleclough C., Cooper D., Perry R. P. Rearrangement of immunoglobulin heavy chain genes during B-lymphocyte development as revealed by studies of mouse plasmacytoma cells. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1422–1426. doi: 10.1073/pnas.77.3.1422. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cunningham A. J., Smith J. B., Mercer E. H. Antibody formation by single cells from lymph nodes and efferent lymph of sheep. J Exp Med. 1966 Oct 1;124(4):701–714. doi: 10.1084/jem.124.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eichmann K. Expression and function of idiotypes of lymphocytes. Adv Immunol. 1978;26:195–254. doi: 10.1016/s0065-2776(08)60231-x. [DOI] [PubMed] [Google Scholar]
- Honjo T., Kataoka T. Organization of immunoglobulin heavy chain genes and allelic deletion model. Proc Natl Acad Sci U S A. 1978 May;75(5):2140–2144. doi: 10.1073/pnas.75.5.2140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
- Lonai P., Ben-Neriah Y., Steinman L., Givol D. Selective participation of immunoglobulin V region and major histocompatibility complex products in antigen binding by T cells. Eur J Immunol. 1978 Dec;8(12):827–832. doi: 10.1002/eji.1830081202. [DOI] [PubMed] [Google Scholar]
- Meo T., Johnson J., Beechey C. V., Andrews S. J., Peters J., Searle A. G. Linkage analyses of murine immunoglobulin heavy chain and serum prealbumin genes establish their location on chromosome 12 proximal to the T (5;12) 31H breakpoint in band 12F1. Proc Natl Acad Sci U S A. 1980 Jan;77(1):550–553. doi: 10.1073/pnas.77.1.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Owen F. L., Finnegan A., Gates E. R., Gottlieb P. D. A mature T lymphocyte subpopulation marker closely linked to the Ig-1 allotype Ch locus. Eur J Immunol. 1979 Dec;9(12):948–955. doi: 10.1002/eji.1830091208. [DOI] [PubMed] [Google Scholar]
- Owen F. L. Polyclonal activation of Ts cells with antiserum directed against an IGH-1 linked candidate for a T-cell receptor constant region marker. J Supramol Struct. 1980;14(2):175–182. doi: 10.1002/jss.400140206. [DOI] [PubMed] [Google Scholar]
- Pisetsky D. S., Riordan S. E., Sachs D. H. Genetic control of the immune response to staphylococcal nuclease. IX. Recombination between genes determining BALB/c antinuclease idiotypes and the heavy chain allotype locus. J Immunol. 1979 Mar;122(3):842–846. [PubMed] [Google Scholar]
- Riblet R., Claflin L., Gibson D. M., Mathieson B. J., Weigert M. Antibody gene linkage studies in (NZB X C58) recombinant-inbred lines. J Immunol. 1980 Feb;124(2):787–789. [PubMed] [Google Scholar]
- Rubin B., Hertel-Wulff B., Kimura A. Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype. J Exp Med. 1979 Aug 1;150(2):307–321. doi: 10.1084/jem.150.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Subbarao B., Ahmed A., Paul W. E., Scher I., Lieberman R., Mosier D. E. Lyb-7, a new B cell alloantigen controlled by genes linked to the IgCH locus. J Immunol. 1979 Jun;122(6):2279–2285. [PubMed] [Google Scholar]
- Taylor B. A., Bailey D. W., Cherry M., Riblet R., Weigert M. Genes for immunoglobulin heavy chain and serum prealbumin protein are linked in mouse. Nature. 1975 Aug 21;256(5519):644–646. doi: 10.1038/256644a0. [DOI] [PubMed] [Google Scholar]
- Taylor B. A., Meier H., Myers D. D. Host-gene control of C-type RNA tumor virus: inheritance of the group-specific antigen of murine leukemia virus. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3190–3194. doi: 10.1073/pnas.68.12.3190. [DOI] [PMC free article] [PubMed] [Google Scholar]