Abstract
A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV used. The susceptibility of bone marrow cells from different strains of mice to A-MuLV-induced transformation can be estimated using the agarose assay. Strains with bone marrow cells of high, medium, and low susceptibility to A-MuLV can be identified. The assay has been used to study the susceptibility of cells from lymphoid organs of fetal and adult mice to A-MuLV. Cell suspensions from fetal liver, adult bone marrow, and adult spleen are susceptible to A-MuLV, while thymocytes are resistant to A-MuLV-induced transformation. Bovine serum albumin gradient fractionation of bone marrow cells before infection with A-MuLV demonstrates that the majority of A-MuLV-sensitive cells are recovered in a broad band partially overlapping the majority of the nucleated cells. The agarose assay system allows study of A-MuLV-lymphoid cell interaction at the level of single cell-single virus particle interaction.
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- Abelson H. T., Rabstein L. S. Lymphosarcoma: virus-induced thymic-independent disease in mice. Cancer Res. 1970 Aug;30(8):2213–2222. [PubMed] [Google Scholar]
- Dicke K. A., Tridente G., van Bekkum D. W. The selective elimination of immunologically competent cells from bone marrow and lymphocyte cell mixtures. 3. In vitro test for detection of immunocompetent cells in fractionated mouse spleen cell suspensions and primate bone marrow suspensions. Transplantation. 1969 Oct;8(4):422–434. doi: 10.1097/00007890-196910000-00014. [DOI] [PubMed] [Google Scholar]
- Kung P. C., Siverstone A. E., McCaffrey R. P., Baltimore D. Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone. J Exp Med. 1975 Apr 1;141(4):855–865. [PMC free article] [PubMed] [Google Scholar]
- MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
- Melchers F., Von Boehmer H., Phillips R. A. B-lymphocyte subpopulations in the mouse. Organ distribution and ontogeny of immunoglobulin-synthesizing and of mitogen-sensitive cells. Transplant Rev. 1975;25:26–58. doi: 10.1111/j.1600-065x.1975.tb00725.x. [DOI] [PubMed] [Google Scholar]
- Metcalf D., Warner N. L., Nossal G. J., Miller J. F., Shortman K., Rabellino E. Growth of B lymphocyte colonies in vitro from mouse lymphoid organs. Nature. 1975 Jun 19;255(5510):630–632. doi: 10.1038/255630a0. [DOI] [PubMed] [Google Scholar]
- Osmond D. G., Nossal G. J. Differentiation of lymphocytes in mouse bone marrow. II. Kinetics of maturation and renewal of antiglobulin-binding cells studied by double labeling. Cell Immunol. 1974 Jul;13(1):132–145. doi: 10.1016/0008-8749(74)90233-0. [DOI] [PubMed] [Google Scholar]
- Phillips R. A., Miller R. G. Marrow environment not required for differentiation of B lymphocytes. Nature. 1974 Oct 4;251(5474):444–446. doi: 10.1038/251444a0. [DOI] [PubMed] [Google Scholar]
- Potter M., Sklar M. D., Rowe W. P. Rapid viral induction of plasmacytomas in pristane-primed BALB-c mice. Science. 1973 Nov 9;182(4112):592–594. doi: 10.1126/science.182.4112.592. [DOI] [PubMed] [Google Scholar]
- Premkumar E., Potter M., Singer P. A., Sklar M. D. Synthesis, surface deposition, and secretion of immunoglobulins by Abelson virus-transformed lymphosarcoma cell lines. Cell. 1975 Oct;6(2):149–159. doi: 10.1016/0092-8674(75)90005-7. [DOI] [PubMed] [Google Scholar]
- Raschke W. C., Ralph P., Watson J., Sklar M., Coon H. Oncogenic transformation of murine lymphoid cells by in vitro infection with Abelson leukemia virus. J Natl Cancer Inst. 1975 May;54(5):1249–1253. doi: 10.1093/jnci/54.5.1249. [DOI] [PubMed] [Google Scholar]
- Rosenberg N., Baltimore D., Scher C. D. In vitro transformation of lymphoid cells by Abelson murine leukemia virus. Proc Natl Acad Sci U S A. 1975 May;72(5):1932–1936. doi: 10.1073/pnas.72.5.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
- Scher C. D., Siegler R. Direct transformation of 3T3 cells by Abelson murine leukaemia virus. Nature. 1975 Feb 27;253(5494):729–731. doi: 10.1038/253729a0. [DOI] [PubMed] [Google Scholar]
- Shevach E. M., Stobo J. D., Green I. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J Immunol. 1972 May;108(5):1146–1151. [PubMed] [Google Scholar]
- Siegler R., Zajdel S. Pathogenesis of Abelson-virus-induced murine leukemia. J Natl Cancer Inst. 1972 Jan;48(1):189–218. [PubMed] [Google Scholar]
- Sklar M. D., Shevach E. M., Green I., Potter M. Transplantation and preliminary characterisation of lymphocyte surface markers of Abelson virus-induced lymphomas. Nature. 1975 Feb 13;253(5492):550–552. doi: 10.1038/253550a0. [DOI] [PubMed] [Google Scholar]
- Sklar M. D., White B. J., Rowe W. P. Initiation of oncogenic transformation of mouse lymphocytes in vitro by Abelson leukemia virus. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4077–4081. doi: 10.1073/pnas.71.10.4077. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Toyoshima K., Vogt P. K. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions. Virology. 1969 Jul;38(3):414–426. doi: 10.1016/0042-6822(69)90154-8. [DOI] [PubMed] [Google Scholar]
- Tyan M. L., Herzenberg L. A. Studies on the ontogeny of the mouse immune system. II. Immunoglobulin-producing cells. J Immunol. 1968 Sep;101(3):446–450. [PubMed] [Google Scholar]