Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Dec;112(3):1275–1279. doi: 10.1128/jb.112.3.1275-1279.1972

R Factor-Controlled Restriction and Modification of Deoxyribonucleic Acid: Restriction Mutants

Robert Yoshimori a,1,2, Daisy Roulland-Dussoix a, Herbert W Boyer a
PMCID: PMC251559  PMID: 4565538

Abstract

Restriction mutants of two different R factor-controlled host specificities (RI and RII) were isolated. All of the restriction mutants examined had a normal modification phenotype. No complementation was observed between the RI and RII host specificities. It is concluded that for each host specificity no protein subunit is shared by the restriction endonuclease and modification methylase.

Full text

PDF
1278

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W., DUSSOIX D. Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage lambda. J Mol Biol. 1962 Jul;5:18–36. doi: 10.1016/s0022-2836(62)80058-8. [DOI] [PubMed] [Google Scholar]
  2. ARBER W., MORSE M. L. HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI. VI. EFFECTS ON BACTERIAL CONJUGATION. Genetics. 1965 Jan;51:137–148. doi: 10.1093/genetics/51.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arber W., Linn S. DNA modification and restriction. Annu Rev Biochem. 1969;38:467–500. doi: 10.1146/annurev.bi.38.070169.002343. [DOI] [PubMed] [Google Scholar]
  4. Arber W., Wauters-Willems D. Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T-. Mol Gen Genet. 1970;108(3):203–217. doi: 10.1007/BF00283350. [DOI] [PubMed] [Google Scholar]
  5. BOYER H. GENETIC CONTROL OF RESTRICTION AND MODIFICATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1652–1660. doi: 10.1128/jb.88.6.1652-1660.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bannister D., Glover S. W. Restriction and modification of bacteriophages by R+ strains of Escherichia coli K12. Biochem Biophys Res Commun. 1968 Mar 27;30(6):735–738. doi: 10.1016/0006-291x(68)90575-5. [DOI] [PubMed] [Google Scholar]
  7. Bannister D., Glover S. W. The isolation and properties of non-restricting mutants of two different host specificities associated with drug resistance factors. J Gen Microbiol. 1970 Apr;61(1):63–71. doi: 10.1099/00221287-61-1-63. [DOI] [PubMed] [Google Scholar]
  8. Boyer H. W. DNA restriction and modification mechanisms in bacteria. Annu Rev Microbiol. 1971;25:153–176. doi: 10.1146/annurev.mi.25.100171.001101. [DOI] [PubMed] [Google Scholar]
  9. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  10. Colson A. M., Colson C., Van Pel A. Host-controlled restriction mutants of Salmonella typhimurium. J Gen Microbiol. 1969 Sep;58(1):57–64. doi: 10.1099/00221287-58-1-57. [DOI] [PubMed] [Google Scholar]
  11. Colson C., Glover S. W., Symonds N., Stacey K. A. The location of the genes for host-controlled modification and restriction in Escherichia coli K-12. Genetics. 1965 Nov;52(5):1043–1050. doi: 10.1093/genetics/52.5.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Falkow S., Citarella R. V., Wohlhieter J. A. The molecular nature of R-factors. J Mol Biol. 1966 May;17(1):102–116. doi: 10.1016/s0022-2836(66)80097-9. [DOI] [PubMed] [Google Scholar]
  13. Glover S. W. Functional analysis of host-specificity mutants in Escherichia coli. Genet Res. 1970 Apr;15(2):237–250. doi: 10.1017/s0016672300001567. [DOI] [PubMed] [Google Scholar]
  14. Hubacek J., Glover S. W. Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli. J Mol Biol. 1970 May 28;50(1):111–127. doi: 10.1016/0022-2836(70)90108-7. [DOI] [PubMed] [Google Scholar]
  15. Lederberg S. Genetics of host-controlled restriction and modification of deoxyribonucleic acid in Escherichia coli. J Bacteriol. 1966 Mar;91(3):1029–1036. doi: 10.1128/jb.91.3.1029-1036.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Meselson M., Yuan R. DNA restriction enzyme from E. coli. Nature. 1968 Mar 23;217(5134):1110–1114. doi: 10.1038/2171110a0. [DOI] [PubMed] [Google Scholar]
  17. Roulland-Dussoix D., Boyer H. W. The Escherichia coli B restriction endonuclease. Biochim Biophys Acta. 1969 Nov 19;195(1):219–229. doi: 10.1016/0005-2787(69)90618-2. [DOI] [PubMed] [Google Scholar]
  18. SCAIFE J., GROSS J. D. Inhibition of multiplication of an Flac factor in Hfr cells of Escherichia coli K-12. Biochem Biophys Res Commun. 1962 May 11;7:403–407. doi: 10.1016/0006-291x(62)90324-8. [DOI] [PubMed] [Google Scholar]
  19. WATANABE T., NISHIDA H., OGATA C., ARAI T., SATO S. EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE IN ENTEROBACTERIACEAE. VII. TWO TYPES OF NATURALLY OCCURRING R FACTORS. J Bacteriol. 1964 Sep;88:716–726. doi: 10.1128/jb.88.3.716-726.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wood W. B. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J Mol Biol. 1966 Mar;16(1):118–133. doi: 10.1016/s0022-2836(66)80267-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES