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. 1970 Sep;67(1):99–106. doi: 10.1073/pnas.67.1.99

Peptide Chain Termination with Mammalian Release Factor

J L Goldstein 1,2,*, A L Beaudet 1,2, C T Caskey 1,2
PMCID: PMC283173  PMID: 4917818

Abstract

We report a method for the in vitro study of peptide chain termination in mammals. A proteinaceous release factor has been isolated from rabbit reticulocyte extracts. This factor and a polyribonucleotide template containing the bases U and A stimulate the release of N-formylmethionine from mammalian [N-formylmethionyl-tRNAf·ribosome] intermediates. Our studies suggest that UAA is a terminator codon for mammalian cells.

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Selected References

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