Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Jul;78(7):4549–4551. doi: 10.1073/pnas.78.7.4549

Distinct forms of both alpha and beta subunits are present in the human Ia molecular pool.

R S Accolla, N Gross, S Carrel, G Corte
PMCID: PMC319829  PMID: 6945597

Abstract

Two distinct subsets of human Ia molecules, called NG1 and NG2, present in all individuals irrespective of their HLA-DR phenotype, which were previously defined by their reactivity with two monoclonal hybridoma antibodies, D1--12 and D4--22, were analyzed by two-dimensional peptide mapping techniques. Results show that, in the Ia molecular pool from a single individual, small beta subunits of the NG1 and NG2 subsets display significant differences from each other. In addition, beta subunits of the same subset from two different allotype Ia molecular pools are also different from each other, thus indicating that NG1 and NG2 subsets carry polymorphic specificities. Moreover, large alpha chains of NG1 and NG2 subsets are different from each other; however, no significant differences are observed in alpha chains of the same subset when different allotype Ia pools are analyzed. The possible genetic implications of these findings are discussed.

Full text

PDF
4549

Images in this article

4550Image
on p.4550
4550Image
on p.4550

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodmer W. F., Jones E. A., Barnstable C. J., Bodmer J. G. Genetics HLA: the major human histocompatibility system. Proc R Soc Lond B Biol Sci. 1978 Jun 5;202(1146):93–116. doi: 10.1098/rspb.1978.0059. [DOI] [PubMed] [Google Scholar]
  2. Carrel S., Tosi R., Gross N., Tanigaki N., Carmagnola A. L., Accolla R. S. Subsets of human Ia-like molecules defined by monoclonal antibodies. Mol Immunol. 1981 May;18(5):403–411. doi: 10.1016/0161-5890(81)90102-4. [DOI] [PubMed] [Google Scholar]
  3. Cook R. G., Vitetta E. S., Uhr J. W., Klein J., Wilde C. E., 3rd, Capra J. D. Structural studies on protein products of murine chromosome 17. III. Partial amino acid sequence of an H-2Kq molecule. J Immunol. 1978 Sep;121(3):1015–1019. [PubMed] [Google Scholar]
  4. Corte G., Damiani G., Calabi F., Fabbi M., Bargellesi A. Analysis of HLA-DR polymorphism by two-dimensional peptide mapping. Proc Natl Acad Sci U S A. 1981 Jan;78(1):534–538. doi: 10.1073/pnas.78.1.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Corte G., Tonda P., Cosulich E., Milstein C. P., Bargellesi A., Ferrarini M. Characterization of IgD. I. Isolation of two molecular forms from human serum. Scand J Immunol. 1979;9(2):141–149. doi: 10.1111/j.1365-3083.1979.tb02716.x. [DOI] [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Humphreys R. E., McCune J. M., Chess L., Herrman H. C., Malenka D. J., Mann D. L., Parham P., Schlossman S. F., Strominger J. L. Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen. J Exp Med. 1976 Jul 1;144(1):98–112. doi: 10.1084/jem.144.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klareskog L., Rask L., Fohlman J., Peterson P. A. Heavy HLA-DR (Ia) antigen chain is controlled by the MHC region. Nature. 1978 Oct 26;275(5682):762–764. doi: 10.1038/275762a0. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Mole L. R. A genetic marker in the variable region of rabbit immunoglobulin heavy chain. Biochem J. 1975 Nov;151(2):351–359. doi: 10.1042/bj1510351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shackelford D. A., Strominger J. L. Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis. J Exp Med. 1980 Jan 1;151(1):144–165. doi: 10.1084/jem.151.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Snary D., Barnstable C. J., Bodmer W. F., Goodfellow P. N., Crumpton M. J. Cellular distrubtion, purification, and molecular nature of human Ia antigens. Scand J Immunol. 1977;6(5):439–452. doi: 10.1111/j.1365-3083.1977.tb02101.x. [DOI] [PubMed] [Google Scholar]
  13. Tanigaki N., Tosi R., Pressman D., Ferrara G. B. Molecular identification of human Ia antigens coded for by a gene locus closely linked to HLA-DR locus. Immunogenetics. 1980;10(2):151–167. doi: 10.1007/BF01561564. [DOI] [PubMed] [Google Scholar]
  14. Tosi R., Tanigaki N., Centis D., Ferrara G. B., Pressman D. Immunological dissection of human Ia molecules. J Exp Med. 1978 Dec 1;148(6):1592–1611. doi: 10.1084/jem.148.6.1592. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES