Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Aug;78(8):4782–4785. doi: 10.1073/pnas.78.8.4782

cDNA sequence of a new chicken embryonic rho-globin.

I B Roninson, V M Ingram
PMCID: PMC320247  PMID: 6170981

Abstract

In order to use specific DNA probes for the study of developmentally regulated gene expression, we have prepared cDNA clones corresponding to chicken embryonic globins by inserting cDNA.mRNA hybrids into the Pst I site of the plasmid pBR322 by using poly(dG) and poly(dC) linkers. The nucleotide sequence of the insert of one clone, representing a nearly full-length copy of an embryonic beta-like globin cDNA, has been determined. The amino acid sequence of the globin encoded by this insert is identical to the sequence of embryonic rho-globin, except for four amino acid residues near the carboxy terminus. Comparison of mRNA sequences of the embryonic and adult chicken beta-globins indicates the presence of extensive deletions in the 3' untranslated region of the embryonic gene.

Full text

PDF

Images in this article

4783Image
on p.4783
4783Image
on p.4783

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger S. L., Birkenmeier C. S. Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes. Biochemistry. 1979 Nov 13;18(23):5143–5149. doi: 10.1021/bi00590a018. [DOI] [PubMed] [Google Scholar]
  2. Berkner K. L., Folk W. R. Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA. J Biol Chem. 1977 May 25;252(10):3176–3184. [PubMed] [Google Scholar]
  3. Brown J. L., Ingram V. M. Structural studies on chick embryonic hemoglobins. J Biol Chem. 1974 Jun 25;249(12):3960–3972. [PubMed] [Google Scholar]
  4. Brutlag D., Fry K., Nelson T., Hung P. Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA. Cell. 1977 Mar;10(3):509–519. doi: 10.1016/0092-8674(77)90038-1. [DOI] [PubMed] [Google Scholar]
  5. Chang A. C., Nunberg J. H., Kaufman R. J., Erlich H. A., Schimke R. T., Cohen S. N. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature. 1978 Oct 19;275(5681):617–624. doi: 10.1038/275617a0. [DOI] [PubMed] [Google Scholar]
  6. Chapman B. S., Tobin A. J., Hood L. E. Complete amino acid sequence of the major early embryonic beta-like globin in chickens. J Biol Chem. 1981 Jun 10;256(11):5524–5531. [PubMed] [Google Scholar]
  7. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  8. Deacon N. J., Shine J., Naora H. Complete nucleotide sequence of a cloned chicken alpha-globin cDNA. Nucleic Acids Res. 1980 Mar 25;8(6):1187–1199. doi: 10.1093/nar/8.6.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dodgson J. B., Strommer J., Engel J. D. Isolation of the chicken beta-globin gene and a linked embryonic beta-like globin gene from a chicken DNA recombinant library. Cell. 1979 Aug;17(4):879–887. doi: 10.1016/0092-8674(79)90328-3. [DOI] [PubMed] [Google Scholar]
  10. Dolan M., Sugarman B. J., Dodgson J. B., Engel J. D. Chromosomal arrangement of the chicken beta-type globin genes. Cell. 1981 Jun;24(3):669–677. doi: 10.1016/0092-8674(81)90093-3. [DOI] [PubMed] [Google Scholar]
  11. Donelson J. E., Wu R. Nucleotide sequence analysis of deoxyribonucleic acid. VI. Determination of 3'-terminal dnucleotide sequences of several species of duplex deoxyribonucleic acid using Escherichia coli deoxyribonucleic acid polymerase I. J Biol Chem. 1972 Jul 25;247(14):4654–4660. [PubMed] [Google Scholar]
  12. Efstratiadis A., Kafatos F. C., Maxam A. M., Maniatis T. Enzymatic in vitro synthesis of globin genes. Cell. 1976 Feb;7(2):279–288. doi: 10.1016/0092-8674(76)90027-1. [DOI] [PubMed] [Google Scholar]
  13. Efstratiadis A., Posakony J. W., Maniatis T., Lawn R. M., O'Connell C., Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M., Slightom J. L. The structure and evolution of the human beta-globin gene family. Cell. 1980 Oct;21(3):653–668. doi: 10.1016/0092-8674(80)90429-8. [DOI] [PubMed] [Google Scholar]
  14. Engel J. D., Dodgson J. B. Analysis of the adult and embryonic chicken globin genes in chromosomal DNA. J Biol Chem. 1978 Nov 25;253(22):8239–8246. [PubMed] [Google Scholar]
  15. Engel J. D., Dodgson J. B. Analysis of the closely linked adult chicken alpha-globin genes in recombinant DNAs. Proc Natl Acad Sci U S A. 1980 May;77(5):2596–2600. doi: 10.1073/pnas.77.5.2596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Farrell R., Gunsalus I. C., Crawford I. P., Johnston J. B., Ito J. Restriction endonuclease sites and aromatic metabolic plasmid structure. Biochem Biophys Res Commun. 1978 May 30;82(2):411–416. doi: 10.1016/0006-291x(78)90891-4. [DOI] [PubMed] [Google Scholar]
  17. Fritsch E. F., Lawn R. M., Maniatis T. Molecular cloning and characterization of the human beta-like globin gene cluster. Cell. 1980 Apr;19(4):959–972. doi: 10.1016/0092-8674(80)90087-2. [DOI] [PubMed] [Google Scholar]
  18. Jones C. W., Kafatos F. C. Structure, organization and evolution of developmentally regulated chorion genes in a silkmoth. Cell. 1980 Dec;22(3):855–867. doi: 10.1016/0092-8674(80)90562-0. [DOI] [PubMed] [Google Scholar]
  19. Lacy E., Hardison R. C., Quon D., Maniatis T. The linkage arrangement of four rabbit beta-like globin genes. Cell. 1979 Dec;18(4):1273–1283. doi: 10.1016/0092-8674(79)90238-1. [DOI] [PubMed] [Google Scholar]
  20. Lauer J., Shen C. K., Maniatis T. The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions. Cell. 1980 May;20(1):119–130. doi: 10.1016/0092-8674(80)90240-8. [DOI] [PubMed] [Google Scholar]
  21. Longacre S. S., Rutter W. J. Isolation of chicken hemoglobin mRNA and synthesis of complementary DNA. J Biol Chem. 1977 Apr 25;252(8):2742–2752. [PubMed] [Google Scholar]
  22. Matsuda G., Maita T., Mizuno K., Ota H. Amino acid sequence of a beta chain of AII component of adult chicken haemoglobin. Nat New Biol. 1973 Aug 22;244(138):244–244. doi: 10.1038/newbio244244a0. [DOI] [PubMed] [Google Scholar]
  23. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  24. Michelson A. M., Orkin S. H. The 3' untranslated regions of the duplicated human alpha-globin genes are unexpectedly divergent. Cell. 1980 Nov;22(2 Pt 2):371–377. doi: 10.1016/0092-8674(80)90347-5. [DOI] [PubMed] [Google Scholar]
  25. Richards R. I., Shine J., Ullrich A., Wells J. R., Goodman H. M. Molecular cloning and sequence analysis of adult chicken betal globin cDNA. Nucleic Acids Res. 1979 Nov 10;7(5):1137–1146. doi: 10.1093/nar/7.5.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Richardson C., Cappello J., Cochran M. D., Armentrout R. W., Brown R. D. Partial sequence analysis of Xenopus alpha- and beta-globin mRNA as determined from recombinant DNA plasmids. Dev Biol. 1980 Jul;78(1):161–172. doi: 10.1016/0012-1606(80)90326-7. [DOI] [PubMed] [Google Scholar]
  27. Roychoudhury R., Jay E., Wu R. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 1976 Jan;3(1):101–116. doi: 10.1093/nar/3.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schaffner W., Kunz G., Daetwyler H., Telford J., Smith H. O., Birnstiel M. L. Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing. Cell. 1978 Jul;14(3):655–671. doi: 10.1016/0092-8674(78)90249-0. [DOI] [PubMed] [Google Scholar]
  29. Seeburg P. H., Shine J., Martial J. A., Ullrich A., Baxter J. D., Goodman H. M. Nucleotide sequence of part of the gene for human chorionic somatomammotropin: purification of DNA complementary to predominant mRNA species. Cell. 1977 Sep;12(1):157–165. doi: 10.1016/0092-8674(77)90193-3. [DOI] [PubMed] [Google Scholar]
  30. Slightom J. L., Blechl A. E., Smithies O. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes. Cell. 1980 Oct;21(3):627–638. doi: 10.1016/0092-8674(80)90426-2. [DOI] [PubMed] [Google Scholar]
  31. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  32. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  33. Tonegawa S., Hozumi N., Matthyssens G., Schuller R. Somatic changes in the content and context of immunoglobulin genes. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):877–889. doi: 10.1101/sqb.1977.041.01.097. [DOI] [PubMed] [Google Scholar]
  34. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. doi: 10.1073/pnas.76.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Wahli W., Dawid I. B. Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1437–1441. doi: 10.1073/pnas.77.3.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Zain S., Sambrook J., Roberts R. J., Keller W., Fried M., Dunn A. R. Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA. Cell. 1979 Apr;16(4):851–861. doi: 10.1016/0092-8674(79)90100-4. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES