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. 1980 Jul 11;8(13):2885–2894. doi: 10.1093/nar/8.13.2885

The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.

M Houghton, M A Easton, A G Stewart, J C Smith, S M Doel, G H Catlin, H M Lewis, T P Patel, J S Emtage, N H Carey, A G Porter
PMCID: PMC324132  PMID: 6159580

Abstract

Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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