Abstract
The molecular cloning and nucleotide sequence analysis of adult chicken beta globin mRNA is reported. DNA sequences derived from in vitro transcrption of globin mRNA were purified and amplified as recombinant DNA using the plasmid pBR322. Sequence analysis of several clones coding for beta globin strongly suggests that transcription errors may be generated near the 5' end of transcripts in vitro by reverse transcription. The complete sequence of the longest beta globin insert containing 51 bases of the 5' untranslated region as well as the complete coding and 3' untranslated regions has been determined.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baralle F. E., Brownlee G. G. AUG is the only recognisable signal sequence in the 5' non-coding regions of eukaryotic mRNA. Nature. 1978 Jul 6;274(5666):84–87. doi: 10.1038/274084a0. [DOI] [PubMed] [Google Scholar]
- Baralle F. E. Complete nucleotide sequence of the 5' noncoding region of rabbit beta-globin mRNA. Cell. 1977 Apr;10(4):549–558. doi: 10.1016/0092-8674(77)90088-5. [DOI] [PubMed] [Google Scholar]
- Baralle F. Complete nucleotide sequence of the 5' noncoding region of human alpha-and beta-globin mRNA. Cell. 1977 Dec;12(4):1085–1095. doi: 10.1016/0092-8674(77)90171-4. [DOI] [PubMed] [Google Scholar]
- Crawford R. J., Scott A. C., Wells J. R. Organization of sequences of avian globin mRNA. Eur J Biochem. 1977 Jan;72(2):291–299. doi: 10.1111/j.1432-1033.1977.tb11252.x. [DOI] [PubMed] [Google Scholar]
- Crawford R. J., Wells J. R. Short half-life precursor of globin messenger RNA from chicken erythroblasts. Biochemistry. 1978 May 2;17(9):1591–1596. doi: 10.1021/bi00602a002. [DOI] [PubMed] [Google Scholar]
- Cummings I. W., Liu A. Y., Salser W. A. Identification of a new chicken alpha-globin structural gene by complementary DNA cloning. Nature. 1978 Nov 23;276(5686):418–419. doi: 10.1038/276418a0. [DOI] [PubMed] [Google Scholar]
- Donelson J. E., Wu R. Nucleotide sequence analysis of deoxyribonucleic acid. VI. Determination of 3'-terminal dnucleotide sequences of several species of duplex deoxyribonucleic acid using Escherichia coli deoxyribonucleic acid polymerase I. J Biol Chem. 1972 Jul 25;247(14):4654–4660. [PubMed] [Google Scholar]
- Efstratiadis A., Kafatos F. C., Maniatis T. The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell. 1977 Apr;10(4):571–585. doi: 10.1016/0092-8674(77)90090-3. [DOI] [PubMed] [Google Scholar]
- Goodman M., Moore G. W., Matsuda G. Darwinian evolution in the genealogy of haemoglobin. Nature. 1975 Feb 20;253(5493):603–608. doi: 10.1038/253603a0. [DOI] [PubMed] [Google Scholar]
- Kafatos F. C., Efstratiadis A., Forget B. G., Weissman S. M. Molecular evolution of human and rabbit beta-globin mRNAs. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5618–5622. doi: 10.1073/pnas.74.12.5618. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lo S. C., Aft R., Ross J., Mueller G. C. Control of globin gene expression by steroid hormones in differentiating Friend leukemia cells. Cell. 1978 Oct;15(2):447–453. doi: 10.1016/0092-8674(78)90014-4. [DOI] [PubMed] [Google Scholar]
- Marotta C. A., Wilson J. T., Forget B. G., Weissman S. M. Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA. J Biol Chem. 1977 Jul 25;252(14):5040–5053. [PubMed] [Google Scholar]
- Martial J. A., Hallewell R. A., Baxter J. D., Goodman H. M. Human growth hormone: complementary DNA cloning and expression in bacteria. Science. 1979 Aug 10;205(4406):602–607. doi: 10.1126/science.377496. [DOI] [PubMed] [Google Scholar]
- Matsuda G., Maita T., Mizuno K., Ota H. Amino acid sequence of a beta chain of AII component of adult chicken haemoglobin. Nat New Biol. 1973 Aug 22;244(138):244–244. doi: 10.1038/newbio244244a0. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Proudfoot N. J. Complete 3' noncoding region sequences of rabbit and human beta-globin messenger RNAs. Cell. 1977 Apr;10(4):559–570. doi: 10.1016/0092-8674(77)90089-7. [DOI] [PubMed] [Google Scholar]
- Russell G. J., Walker P. M., Elton R. A., Subak-Sharpe J. H. Doublet frequency analysis of fractionated vertebrate nuclear DNA. J Mol Biol. 1976 Nov;108(1):1–23. doi: 10.1016/s0022-2836(76)80090-3. [DOI] [PubMed] [Google Scholar]
- Seeburg P. H., Shine J., Martial J. A., Baxter J. D., Goodman H. M. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature. 1977 Dec 8;270(5637):486–494. doi: 10.1038/270486a0. [DOI] [PubMed] [Google Scholar]
- Sinclair J. H., Brown D. D. Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics. Biochemistry. 1971 Jul 6;10(14):2761–2769. doi: 10.1021/bi00790a017. [DOI] [PubMed] [Google Scholar]
- Weatherall D. J., Clegg J. B. Recent developments in the molecular genetics of human hemoglobin. Cell. 1979 Mar;16(3):467–479. doi: 10.1016/0092-8674(79)90022-9. [DOI] [PubMed] [Google Scholar]
- van den Berg J., van Ooyen A., Mantei N., Schamböck A., Grosveld G., Flavell R. A., Weissmann C. Comparison of cloned rabbit and mouse beta-globin genes showing strong evolutionary divergence of two homologous pairs of introns. Nature. 1978 Nov 2;276(5683):37–44. doi: 10.1038/276037a0. [DOI] [PubMed] [Google Scholar]