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. 1977 Jul;4(7):2389–2406. doi: 10.1093/nar/4.7.2389

Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene.

P Humphries, M Cochet, A Krust, P Gerlinger, P Kourilsky, P Chambon
PMCID: PMC342573  PMID: 333389

Abstract

Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The resultant hybrid plasmids, amplified by transfection of E. coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.

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Selected References

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