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. 1977 Jul;4(7):2389–2406. doi: 10.1093/nar/4.7.2389

Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene.

P Humphries, M Cochet, A Krust, P Gerlinger, P Kourilsky, P Chambon
PMCID: PMC342573  PMID: 333389

Abstract

Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The resultant hybrid plasmids, amplified by transfection of E. coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beard P., Morrow J. F., Berg P. Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease. J Virol. 1973 Dec;12(6):1303–1313. doi: 10.1128/jvi.12.6.1303-1313.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bellard M., Oudet P., Germond J. E., Chambon P. Subunit structure of simian-virus-40 minichromosome. Eur J Biochem. 1976 Nov 15;70(2):543–553. doi: 10.1111/j.1432-1033.1976.tb11046.x. [DOI] [PubMed] [Google Scholar]
  3. Bollum F. J., Chang L. M., Tsiapalis C. M., Dorson J. W. Nucleotide polymerizing enzymes from calf thymus gland. Methods Enzymol. 1974;29:70–81. doi: 10.1016/0076-6879(74)29010-4. [DOI] [PubMed] [Google Scholar]
  4. Brison O., Cambon P. A simple and efficient method to remove ribonuclease contamination from pancreatic deoxyribonuclease preparations. Anal Biochem. 1976 Oct;75(2):402–409. doi: 10.1016/0003-2697(76)90094-4. [DOI] [PubMed] [Google Scholar]
  5. Browne J. K., Paddock G. V., Liu A., Clarke P., Heindell H. C., Salser W. Nucleotide sequences from the rabbit beta globin gene inserted into Escherichia coli plasmids. Science. 1977 Jan 28;195(4276):389–391. doi: 10.1126/science.318762. [DOI] [PubMed] [Google Scholar]
  6. Cheng C. C., Brownlee G. G., Carey N. H., Doel M. T., Gillam S., Smith M. The 3' terminal sequence of chicken ovalbumin messenger RNA and its comparison with other messenger RNA molecules. J Mol Biol. 1976 Nov 15;107(4):527–547. doi: 10.1016/s0022-2836(76)80081-2. [DOI] [PubMed] [Google Scholar]
  7. Covey C., Richardson D., Carbon J. A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acid. Mol Gen Genet. 1976 May 7;145(2):155–158. doi: 10.1007/BF00269587. [DOI] [PubMed] [Google Scholar]
  8. Efstratiadis A., Kafatos F. C., Maxam A. M., Maniatis T. Enzymatic in vitro synthesis of globin genes. Cell. 1976 Feb;7(2):279–288. doi: 10.1016/0092-8674(76)90027-1. [DOI] [PubMed] [Google Scholar]
  9. Higuchi R., Paddock G. V., Wall R., Salser W. A general method for cloning eukaryotic structural gene sequences. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3146–3150. doi: 10.1073/pnas.73.9.3146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hossenlopp P., Oudet P., Chambon P. Animal DNA-dependent RNA polymerases. Studies on the binding of mammalian RNA polymerases AI and B to Simian virus 40 DNA. Eur J Biochem. 1974 Jan 16;41(2):397–411. doi: 10.1111/j.1432-1033.1974.tb03281.x. [DOI] [PubMed] [Google Scholar]
  11. Jackson D. A., Symons R. H., Berg P. Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2904–2909. doi: 10.1073/pnas.69.10.2904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klenow H., Overgaard-Hansen K., Patkar S. A. Proteolytic cleavage fo native DNA polymerase into two different catalytic fragments. Influence of assay condtions on the change of exonuclease activity and polymerase activity accompanying cleavage. Eur J Biochem. 1971 Oct 14;22(3):371–381. doi: 10.1111/j.1432-1033.1971.tb01554.x. [DOI] [PubMed] [Google Scholar]
  13. Lobban P. E., Kaiser A. D. Enzymatic end-to end joining of DNA molecules. J Mol Biol. 1973 Aug 15;78(3):453–471. doi: 10.1016/0022-2836(73)90468-3. [DOI] [PubMed] [Google Scholar]
  14. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  15. Marotta C. A., Forget B. G., Weissman S. M., Verma I. M., McCaffrey R. P., Baltimore D. Nucleotide sequences of human globin messenger RNA. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2300–2304. doi: 10.1073/pnas.71.6.2300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Palmiter R. D. Quantitation of parameters that determine the rate of ovalbumin synthesis. Cell. 1975 Mar;4(3):189–189. doi: 10.1016/0092-8674(75)90167-1. [DOI] [PubMed] [Google Scholar]
  17. Pettersson U., Mulder C., Deluis H., Sharp P. A. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. doi: 10.1073/pnas.70.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Poon R., Paddock G. V., Heindell H., Whitcome P., Salser W., Kacian D., Bank A., Gambino R., Ramirez F. Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3502–3506. doi: 10.1073/pnas.71.9.3502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rougeon F., Kourilsky P., Mach B. Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid. Nucleic Acids Res. 1975 Dec;2(12):2365–2378. doi: 10.1093/nar/2.12.2365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rougeon F., Mach B. Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3418–3422. doi: 10.1073/pnas.73.10.3418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Salser W., Bowen S., Browne D., el-Adli F., Fedoroff N., Fry K., Heindell H., Paddock G., Poon R., Wallace B. Investigation of the organization of mammalian chromosomes at the DNA sequence level. Fed Proc. 1976 Jan;35(1):23–35. [PubMed] [Google Scholar]
  22. Shapiro D. J., Taylor J. M., McKnight G. S., Palacios R., Gonzalez C., Kiely M. L., Schimke R. T. Isolation of hen oviduct ovalbumin and rat live albumin polysomes by indirect immunoprecipitation. J Biol Chem. 1974 Jun 25;249(12):3665–3671. [PubMed] [Google Scholar]
  23. Shenk T. E., Rhodes C., Rigby P. W., Berg P. Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40. Proc Natl Acad Sci U S A. 1975 Mar;72(3):989–993. doi: 10.1073/pnas.72.3.989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Vogt V. M. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur J Biochem. 1973 Feb 15;33(1):192–200. doi: 10.1111/j.1432-1033.1973.tb02669.x. [DOI] [PubMed] [Google Scholar]
  25. Wood K. O., Lee J. C. Integration of synthetic globin genes into an E. coli plasmid. Nucleic Acids Res. 1976 Aug;3(8):1961–1971. doi: 10.1093/nar/3.8.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yang R. C., Van de Voorde A., Fiers W. Cleavage map of the simian-virus-40 genome by the restriction endonuclease III of Haemopholus aegyptius. Eur J Biochem. 1976 Jan 2;61(1):101–117. doi: 10.1111/j.1432-1033.1976.tb10002.x. [DOI] [PubMed] [Google Scholar]

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