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. 1976 Aug;3(8):2101–2113. doi: 10.1093/nar/3.8.2101

Minichromosome of simian virus 40: presence of histone HI.

A J Varshavsky, V V Bakayev, P M Chumackov, G P Georgiev
PMCID: PMC343065  PMID: 184438

Abstract

In contrast to conclusions of previous studies /I-3/ claiming the absence of histone HI from the SV40 and polyoma viral minichromosomes we have found that a preparation of purified SV40 minichromosomes does contain histone HI. The content of HI in relation to other four histones in the SV40 minichromosomes is close to that in the cellular chromatin. Histone HI in the isolated SV40 minichromosomes is bound apparently to internucleosomal DNA stretches as was shown already for HI in the cellular chromatin /4/. In addition it was found that more than 90% of the purified SV40 minichromosomes migrated as a single discrete deoxyribonucleoprotein band upon agarose gel electrophoresis.

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Selected References

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