Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 May;81(9):2640–2644. doi: 10.1073/pnas.81.9.2640

Amino acid sequence of homologous rat atrial peptides: natriuretic activity of native and synthetic forms.

N G Seidah, C Lazure, M Chrétien, G Thibault, R Garcia, M Cantin, J Genest, R F Nutt, S F Brady, T A Lyle, et al.
PMCID: PMC345125  PMID: 6232612

Abstract

A substance called atrial natriuretic factor (ANF), localized in secretory granules of atrial cardiocytes, was isolated as four homologous natriuretic peptides from homogenates of rat atria. The complete sequence of the longest form showed that it is composed of 33 amino acids. The three other shorter forms (2-33, 3-33, and 8-33) represent amino-terminally truncated versions of the 33 amino acid parent molecule as shown by analysis of sequence, amino acid composition, or both. The proposed primary structure agrees entirely with the amino acid composition and reveals no significant sequence homology with any known protein or segment of protein. The short form ANF-(8-33) was synthesized by a multi-fragment condensation approach and the synthetic product was shown to exhibit specific activity comparable to that of the natural ANF-(3-33).

Full text

PDF
2640

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arison B. H., Hirschmann R., Paleveda W. J., Brady S. F., Veber D. F. On the low energy solution conformation of somatostatin. Biochem Biophys Res Commun. 1981 Jun 16;100(3):1148–1153. doi: 10.1016/0006-291x(81)91944-6. [DOI] [PubMed] [Google Scholar]
  2. Currie M. G., Geller D. M., Cole B. R., Boylan J. G., YuSheng W., Holmberg S. W., Needleman P. Bioactive cardiac substances: potent vasorelaxant activity in mammalian atria. Science. 1983 Jul 1;221(4605):71–73. doi: 10.1126/science.6857267. [DOI] [PubMed] [Google Scholar]
  3. Currie M. G., Geller D. M., Cole B. R., Siegel N. R., Fok K. F., Adams S. P., Eubanks S. R., Galluppi G. R., Needleman P. Purification and sequence analysis of bioactive atrial peptides (atriopeptins). Science. 1984 Jan 6;223(4631):67–69. doi: 10.1126/science.6419347. [DOI] [PubMed] [Google Scholar]
  4. Flynn T. G., de Bold M. L., de Bold A. J. The amino acid sequence of an atrial peptide with potent diuretic and natriuretic properties. Biochem Biophys Res Commun. 1983 Dec 28;117(3):859–865. doi: 10.1016/0006-291x(83)91675-3. [DOI] [PubMed] [Google Scholar]
  5. Garcia R., Cantin M., Thibault G., Ong H., Genest J. Relationship of specific granules to the natriuretic and diuretic activity of rat atria. Experientia. 1982 Sep 15;38(9):1071–1073. doi: 10.1007/BF01955373. [DOI] [PubMed] [Google Scholar]
  6. Lazure C., Seidah N. G., Chrétien M., Lallier R., St-Pierre S. Primary structure determination of Escherichia coli heat-stable enterotoxin of porcine origin. Can J Biochem Cell Biol. 1983 May;61(5):287–292. doi: 10.1139/o83-039. [DOI] [PubMed] [Google Scholar]
  7. Lazure C., Seidah N. G., Pélaprat D., Chrétien M. Proteases and posttranslational processing of prohormones: a review. Can J Biochem Cell Biol. 1983 Jul;61(7):501–515. doi: 10.1139/o83-066. [DOI] [PubMed] [Google Scholar]
  8. Seidah N. G., Rochemont J., Hamelin J., Lis M., Chrétien M. Primary structure of the major human pituitary pro-opiomelanocortin NH2-terminal glycopeptide. Evidence for an aldosterone-stimulating activity. J Biol Chem. 1981 Aug 10;256(15):7977–7984. [PubMed] [Google Scholar]
  9. Thibault G., Garcia R., Cantin M., Genest J., Lazure C., Seidah N. G., Chrétien M. Primary structure of a high Mr form of rat atrial natriuretic factor. FEBS Lett. 1984 Feb 27;167(2):352–356. doi: 10.1016/0014-5793(84)80155-6. [DOI] [PubMed] [Google Scholar]
  10. Thibault G., Garcia R., Seidah N. G., Lazure C., Cantin M., Chrétien M., Genest J. Purification of three rat atrial natriuretic factors and their amino acid composition. FEBS Lett. 1983 Dec 12;164(2):286–290. doi: 10.1016/0014-5793(83)80303-2. [DOI] [PubMed] [Google Scholar]
  11. Trippodo N. C., Macphee A. A., Cole F. E., Blakesley H. L. Partial chemical characterization of a natriuretic substance in rat atrial heart tissue. Proc Soc Exp Biol Med. 1982 Sep;170(4):502–508. doi: 10.3181/00379727-170-41465. [DOI] [PubMed] [Google Scholar]
  12. Veber D. F., Holly F. W., Paleveda W. J., Nutt R. F., Bergstrand S. J., Torchiana M., Glitzer M. S., Saperstein R., Hirschmann R. Conformationally restricted bicyclic analogs of somatostatin. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2636–2640. doi: 10.1073/pnas.75.6.2636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. de Bold A. J. Atrial natriuretic factor of the rat heart. Studies on isolation and properties. Proc Soc Exp Biol Med. 1982 Jun;170(2):133–138. doi: 10.3181/00379727-170-41408. [DOI] [PubMed] [Google Scholar]
  14. de Bold A. J., Borenstein H. B., Veress A. T., Sonnenberg H. A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats. Life Sci. 1981 Jan 5;28(1):89–94. doi: 10.1016/0024-3205(81)90370-2. [DOI] [PubMed] [Google Scholar]
  15. de Bold A. J., Flynn T. G. Cardionatrin I - a novel heart peptide with potent diuretic and natriuretic properties. Life Sci. 1983 Jul 18;33(3):297–302. doi: 10.1016/0024-3205(83)90390-9. [DOI] [PubMed] [Google Scholar]
  16. de Bold A. J. Tissue fractionation studies on the relationship between an atrial natriuretic factor and specific atrial granules. Can J Physiol Pharmacol. 1982 Mar;60(3):324–330. doi: 10.1139/y82-045. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES