Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 May;77(5):2757–2761. doi: 10.1073/pnas.77.5.2757

Proportion of fetal hemoglobin synthesis decreases during erythroid cell maturation.

D H Chui, S C Wong, M W Enkin, M Patterson, R A Ives
PMCID: PMC349483  PMID: 6156456

Abstract

Peripheral blood mononuclear cells from pregnant and postpartum women were cultured in vitro with erythropoietin. Burst-forming unit (BFU-E)-derived erythroid colonies composed of immature erythroblasts with low hemoglobin contents were observed by day 8 of culture. By day 12 of culture, numerous BFU-E-derived erythroid colonies with high hemoglobin contents were present. The gamma/(gamma + beta) globin synthetic ratio was approximately 12% in the early cultures and 6% in the late cultures, indicating that the proportion of fetal hemoglobin synthesis decreases during erythroid cell maturation. These studies also reveal that the capacity fof fetal hemoglobin production by peripheral blood BFU-E in vitro is not altered during pregnancy.

Full text

PDF
2757

Images in this article

2759Image
on p.2759
2759Image
on p.2759

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BETKE K., MARTI H. R., SCHLICHT I. Estimation of small percentages of foetal haemoglobin. Nature. 1959 Dec 12;184(Suppl 24):1877–1878. doi: 10.1038/1841877a0. [DOI] [PubMed] [Google Scholar]
  2. Bard H. The postnatal decline of hemoglobin F synthesis in normal full-term infants. J Clin Invest. 1975 Feb;55(2):395–398. doi: 10.1172/JCI107943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyer S. H., Belding T. K., Margolte L., Noyes A. N., Burke P. J., Bell W. R. Variations in the frequency of fetal hemoglobin-bearing erythrocytes (F-cells) in well adults, pregnant women, and adult leukemics. Johns Hopkins Med J. 1975 Sep;137(3):105–115. [PubMed] [Google Scholar]
  4. Chui D. H., Liao S. K., Walker K. Fetal erythropoiesis in steel mutant mice. III. Defect in differentiation from BFU-E to CFU-E during early development. Blood. 1978 Mar;51(3):539–547. [PubMed] [Google Scholar]
  5. Clarke B. J., Housman D. Characterization of an erythroid precursor cell of high proliferative capacity in normal human peripheral blood. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1105–1109. doi: 10.1073/pnas.74.3.1105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clarke B. J., Nathan D. G., Alter B. P., Forget B. G., Hillman D. G., Housman D. Hemoglobin synthesis in human BFU-E and CFU-E-derived erythroid colonies. Blood. 1979 Oct;54(4):805–817. [PubMed] [Google Scholar]
  7. Craig M. L., Southard J. L. Some factors associated with a developmental change in hemoglobin components in early fetal mice. Dev Biol. 1967 Oct;16(4):331–340. doi: 10.1016/0012-1606(67)90046-2. [DOI] [PubMed] [Google Scholar]
  8. Eaves C. J., Eaves A. C. Erythropoietin (Ep) dose-response curves for three classes of erythroid progenitors in normal human marrow and in patients with polycythemia vera. Blood. 1978 Dec;52(6):1196–1210. [PubMed] [Google Scholar]
  9. Efremov C. D., Huisman T. H., Bowman K., Wrightstone R. N., Shroeder W. A. Microchromatography of hemoglobins. II. A rapid method for the determination of hemoglobin A2. J Lab Clin Med. 1974 Apr;83(4):657–664. [PubMed] [Google Scholar]
  10. Efremov G. D., Huisman T. H., Smith L. L., Wilson J. B., Kitchens J. L., Wrightstone R. N., Adams H. R. Hemoglobin Richmond, a human hemoglobin which forms asymmetric hybrids with other hemoglobins. J Biol Chem. 1969 Nov 25;244(22):6105–6116. [PubMed] [Google Scholar]
  11. Fantoni A., De la Chapelle A., Marks P. A. Synthesis of embryonic hemoglobins during erythroid cell development in fetal mice. J Biol Chem. 1969 Feb 25;244(4):675–681. [PubMed] [Google Scholar]
  12. Heath D. S., Axelrad A. A., McLeod D. L., Shreeve M. M. Separation of the erythropoietin-responsive progenitors BFU-E and CFU-E in mouse bone marrow by unit gravity sedimentation. Blood. 1976 May;47(5):777–792. [PubMed] [Google Scholar]
  13. Kidoguchi K., Ogawa M., Karam J. D., Martin A. G. Augmentation of fetal hemoglobin (HbF) synthesis in culture by human erythropoietic precursors in the marrow and peripheral blood: studies in sickle cell anemia and nonhemoglobinopathic adults. Blood. 1978 Dec;52(6):1115–1124. [PubMed] [Google Scholar]
  14. LUND C. J., SISSON T. R. Blood volume and anemia of mother and baby. Am J Obstet Gynecol. 1958 Nov;76(5):1013–1024. doi: 10.1016/0002-9378(58)90184-4. [DOI] [PubMed] [Google Scholar]
  15. Manasc B., Jepson J. Erythropoietin in plasma and urine during human pregnancy. Can Med Assoc J. 1969 Apr 19;100(15):687–691. [PMC free article] [PubMed] [Google Scholar]
  16. Nienhuis A. W., Stamatoyannopoulos G. Hemoglobin switching. Cell. 1978 Sep;15(1):307–315. doi: 10.1016/0092-8674(78)90106-x. [DOI] [PubMed] [Google Scholar]
  17. Ogawa M., Grush O. C., O'Dell R. F., Hara H., MacEachern M. D. Circulating erythropoietic precursors assessed in culture: characterization in normal men and patients with hemoglobinopathies. Blood. 1977 Dec;50(6):1081–1092. [PubMed] [Google Scholar]
  18. Papayannopoulou T., Brice M., Stamatoyannopoulos G. Hemoglobin F synthesis in vitro: evidence for control at the level of primitive erythroid stem cells. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2923–2927. doi: 10.1073/pnas.74.7.2923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Papayannopoulou T., Nakamoto B., Buckley J., Kurachi S., Nute P. E., Stamatoyannopoulos G. Erythroid progenitors circulating in the blood of adult individuals produce fetal hemoglobin in culture. Science. 1978 Mar 24;199(4335):1349–1350. doi: 10.1126/science.628844. [DOI] [PubMed] [Google Scholar]
  20. Papayannopoulou T., Nute P. E., Kurachi S., Stamatoyannopoulos G. Consistent activation of fetal hemoglobin synthesis in cultured adult bone marrow cells. Blood. 1978 Apr;51(4):671–679. [PubMed] [Google Scholar]
  21. Pembrey M. E., Weatherall D. J., Clegg J. B. Maternal synthesis of haemoglobin F in pregnancy. Lancet. 1973 Jun 16;1(7816):1350–1354. doi: 10.1016/s0140-6736(73)91675-9. [DOI] [PubMed] [Google Scholar]
  22. Piomelli S. A micromethod for free erythrocyte porphyrins: the FEP test. J Lab Clin Med. 1973 Jun;81(6):932–940. [PubMed] [Google Scholar]
  23. Popat N., Wood W. G., Weatherall D. J., Turnbull A. C. Pattern of maternal F-cell production during pregnancy. Lancet. 1977 Aug 20;2(8034):377–379. doi: 10.1016/s0140-6736(77)90305-1. [DOI] [PubMed] [Google Scholar]
  24. Stohlman F., Jr, Ebbe S., Morse B., Howard D., Donovan J. Regulation of erythropoiesis. XX. Kinetics of red cell production. Ann N Y Acad Sci. 1968 Mar 29;149(1):156–172. doi: 10.1111/j.1749-6632.1968.tb15149.x. [DOI] [PubMed] [Google Scholar]
  25. Wood W. G. Haemoglobin synthesis during human fetal development. Br Med Bull. 1976 Sep;32(3):282–287. doi: 10.1093/oxfordjournals.bmb.a071376. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES