Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1975 Sep;16(3):498–507. doi: 10.1128/jvi.16.3.498-507.1975

DNA synthesis and DNA polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants.

G M Aron, D J Purifoy, P A Schaffer
PMCID: PMC354696  PMID: 169388

Abstract

Fifteen temperature-sensitive mutants of herpes simplex virus type 1 were studied with regard to the relationship between their ability to synthesize viral DNA and to induce viral DNA polymerase (DP) activity at permissive (34 C) and nonpermissive (39 C) temperatures. At 34 C, all mutants synthesized viral DNA, while at 39 C four mutants demonstrated a DNA+ phenotype, three were DNA+/-, and eight were DNA-. DNA+ mutants induced levels of DP activity similar to thhose of the wild-type virus at both temperatures, and DNA+/- mutants induced reduced levels of DP activity at 39 C but not at 34 C. Among the DNA- mutants three were DP+, two were DP+/-, and three showed reduced DP activity at 34 C with no DP activity at 39 C. DNA-, DP- mutants induced the synthesis of a temperature-sensitive DP as determined by in vivo studies.

Full text

PDF
498

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEN-PORAT T., KAPLAN A. S. The synthesis and fate of pseudorabies virus DNA in infected mammalian cells in the stationary phase of growth. Virology. 1963 Jun;20:310–317. doi: 10.1016/0042-6822(63)90120-x. [DOI] [PubMed] [Google Scholar]
  2. De Waard A., Paul A. V., Lehman I. R. The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241–1248. doi: 10.1073/pnas.54.4.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dreesman G. R., Benyesh-Melnick M. Spectrum of human cytomegalovirus complement-fixing antigens. J Immunol. 1967 Dec;99(6):1106–1114. [PubMed] [Google Scholar]
  4. Esparza J., Purifoy D. J., Schaffer P. A., Benyesh-Melnick M. Isolation, complementation and preliminary phenotypic characterization of temperature-sensitive mutants of herpes simplex virus type 2. Virology. 1974 Feb;57(2):554–565. doi: 10.1016/0042-6822(74)90194-9. [DOI] [PubMed] [Google Scholar]
  5. Halliburton I. W., Timbury M. C. Characterisation of temperature-sensitive mutants of herpes simplex virus type 2. Growth and DNA synthesis. Virology. 1973 Jul;54(1):60–68. doi: 10.1016/0042-6822(73)90114-1. [DOI] [PubMed] [Google Scholar]
  6. Hay J., Perera P. A., Morrison J. M., Gentry G. A., Subak-Sharpe J. H. Herpes virus-specified proteins. In: strategy of the viral genome. Ciba Found Symp. 1971:355–376. [PubMed] [Google Scholar]
  7. KIT S., DUBBS D. R. Acquisition of thymidine kinase activity by herpes simplex-infected mouse fibroblast cells. Biochem Biophys Res Commun. 1963 Apr 2;11:55–59. doi: 10.1016/0006-291x(63)90027-5. [DOI] [PubMed] [Google Scholar]
  8. Karam J. D., Speyer J. F. Reversible inactivation of T4 ts DNA polymerase mutants in vivo. Virology. 1970 Sep;42(1):196–203. doi: 10.1016/0042-6822(70)90252-7. [DOI] [PubMed] [Google Scholar]
  9. Keir H. M., Hay J., Morrison J. M., Subak-Sharpe H. Altered properties of deoxyribonucleic acid nucleotidyltransferase after infection of mammalian cells with herpes simplex virus. Nature. 1966 Apr 23;210(5034):369–371. doi: 10.1038/210369a0. [DOI] [PubMed] [Google Scholar]
  10. Keir H. M., Subak-Sharpe H., Shedden W. I., Watson D. H., Wildy P. Immunological evidence for a specific DNA polymerase produced after infection by herpes simplex virus. Virology. 1966 Sep;30(1):154–157. doi: 10.1016/s0042-6822(66)81022-x. [DOI] [PubMed] [Google Scholar]
  11. Kit S., Piekarski L. J., Dubbs D. R. DNA polymerase induced by Simian virus 40. J Gen Virol. 1967 Apr;1(2):163–173. doi: 10.1099/0022-1317-1-2-163. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Müller W. E., Falke D., Heicke B., Zahn R. K. Biological activity of 2-phenylethanol and its derivatives. 8. Influence on herpesvirus DNA-synthesis in rabbit kidney cells. Arch Gesamte Virusforsch. 1973;40(3):205–214. doi: 10.1007/BF01242539. [DOI] [PubMed] [Google Scholar]
  14. Müller W. E., Falke D., Zahn R. K. DNA-dependent DNA polymerase pattern in noninfected and herpesvirus infected rabbit kidney cells. Arch Gesamte Virusforsch. 1973;42(3):278–284. doi: 10.1007/BF01265653. [DOI] [PubMed] [Google Scholar]
  15. Pringle C. R., Howard D. K., Hay J. Termperature-sensitive mutants of pseudorabies virus with differential effects on viral and host DNA synthesis. Virology. 1973 Oct;55(2):495–505. doi: 10.1016/0042-6822(73)90191-8. [DOI] [PubMed] [Google Scholar]
  16. Schaffer P. A., Aron G. M., Biswal N., Benyesh-Melnick M. Temperature-sensitive mutants of herpes simplex virus type 1: isolation, complementation and partial characterization. Virology. 1973 Mar;52(1):57–71. doi: 10.1016/0042-6822(73)90398-x. [DOI] [PubMed] [Google Scholar]
  17. Schaffer P. A., Brunschwig J. P., McCombs R. M., Benyesh-Melnick M. Electron microscopic studies of temperature-sensitive mutants of herpes simplex virus type 1. Virology. 1974 Dec;62(2):444–457. doi: 10.1016/0042-6822(74)90406-1. [DOI] [PubMed] [Google Scholar]
  18. Schaffer P. A., Courtney R. J., McCombs R. M., Benyesh-Melnick M. A temperature-sensitive mutant of herpes simplex virus defective in glycoprotein synthesis. Virology. 1971 Nov;46(2):356–368. doi: 10.1016/0042-6822(71)90037-7. [DOI] [PubMed] [Google Scholar]
  19. Schaffer P., Vonka V., Lewis R., Benyesh-Melnick M. Temperature-sensitive mutants of herpes simplex virus. Virology. 1970 Dec;42(4):1144–1146. doi: 10.1016/0042-6822(70)90364-8. [DOI] [PubMed] [Google Scholar]
  20. Swartz M. N., Nakamura H., Lehman I. R. Activation of a defective DNA polymerase in a temperature-sensitive mutant of bacteriophage T4. Virology. 1972 Feb;47(2):338–353. doi: 10.1016/0042-6822(72)90269-3. [DOI] [PubMed] [Google Scholar]
  21. Timbury M. C., Subak-Sharpe J. H. Genetic interactions between temperature-sensitive mutants of types 1 and 2 herpes simplex viruses. J Gen Virol. 1973 Mar;18(3):347–357. doi: 10.1099/0022-1317-18-3-347. [DOI] [PubMed] [Google Scholar]
  22. Weissbach A., Hong S. C., Aucker J., Muller R. Characterization of herpes simplex virus-induced deoxyribonucleic acid polymerase. J Biol Chem. 1973 Sep 25;248(18):6270–6277. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES