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. 1976 Oct;20(1):222–233. doi: 10.1128/jvi.20.1.222-233.1976

Physical maps for Herpes simplex virus type 1 DNA for restriction endonucleases Hind III, Hpa-1, and X. bad.

N M Wilkie
PMCID: PMC354983  PMID: 185413

Abstract

It has been proposed that the genome of herpes simplex virus type 1 (HSV-1) consists of two internal unique sequences, S and L, bounded by two sets of redundant sequences (P. Sheldrick and N. Berthelot, 1974). In this arrangement, terminal sequences (TRs and TRl) are repeated in an internal inverted form (IRs and IRl) and delimit S and L. Furthermore, a body of evidence has accumulated that suggests that S and L themselves are inverted, giving rise to four related forms of the HSV genome. In this study the ordering of restruction endonuclease fragments of HSV-1 DNA for physical maps has been studied using molecular hybridization techniques and the cleavage of isolated restriction endonuclease fragments with further restriction endonucleases. Physical maps for the fragments produced by Hind III, Hpa-1, and X. bad have been constructed for the four related forms of the HSV-1 genome. TRs and IRs were found to be between 3.5 x 10(6) and 4.5 x 10(6) daltons, TRl and IRl about 6 x 10(6) daltons, S about 8 x 10(6) to 9 x 10(6) daltons, and L about 6.8 x 10(6) daltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clements J. B., Cortini R., Wilkie N. M. Analysis of herpesvirus DNA substructure by means of restriction endonucleases. J Gen Virol. 1976 Feb;30(2):243–256. doi: 10.1099/0022-1317-30-2-243. [DOI] [PubMed] [Google Scholar]
  2. Grafstrom R. H., Alwine J. C., Steinhart W. L., Hill C. W., Hyman R. W. The terminal repetition of herpes simplex virus DNA. Virology. 1975 Sep;67(1):144–157. doi: 10.1016/0042-6822(75)90412-2. [DOI] [PubMed] [Google Scholar]
  3. Grafstrom R. H., Alwine J. C., Steinhart W. L., Hill C. W. Terminal repetitions in herpes simplex virus type 1 DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):679–681. doi: 10.1101/sqb.1974.039.01.081. [DOI] [PubMed] [Google Scholar]
  4. Hayward G. S., Frenkel N., Roizman B. Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc Natl Acad Sci U S A. 1975 May;72(5):1768–1772. doi: 10.1073/pnas.72.5.1768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hayward G. S., Jacob R. J., Wadsworth S. C., Roizman B. Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4243–4247. doi: 10.1073/pnas.72.11.4243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hyman R. W., Burke S., Kudler L. A nearby inverted repeat of the terminal sequence of herpes simplex virus DNA. Biochem Biophys Res Commun. 1976 Jan 26;68(2):609–615. doi: 10.1016/0006-291x(76)91189-x. [DOI] [PubMed] [Google Scholar]
  7. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Roizman B., Kozak M., Honess R. W., Hayward G. Regulation of herpesvirus macromolecular synthesis: evidence for multilevel regulation of herpes simplex 1 RNA and protein synthesis. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):687–701. doi: 10.1101/sqb.1974.039.01.083. [DOI] [PubMed] [Google Scholar]
  9. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  10. Sheldrick P., Berthelot N. Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):667–678. doi: 10.1101/sqb.1974.039.01.080. [DOI] [PubMed] [Google Scholar]
  11. Skare J., Summers W. P., Summers W. C. Structure and function of herpesvirus genomes. I. comparison of five HSV-1 and two HSV-2 strains by cleavage their DNA with eco R I restriction endonuclease. J Virol. 1975 Apr;15(4):726–732. doi: 10.1128/jvi.15.4.726-732.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  13. Wadsworth S., Hayward G. S., Roizman B. Anatomy of herpes simplex virus DNA. V. Terminally repetitive sequences. J Virol. 1976 Feb;17(2):503–512. doi: 10.1128/jvi.17.2.503-512.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilkie N. M., Clements J. B., Macnab J. C., Subak-Sharpe J. H. The structure and biological properties of herpes simplex virus DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):657–666. doi: 10.1101/sqb.1974.039.01.079. [DOI] [PubMed] [Google Scholar]
  15. Wilkie N. M., Cortini R. Sequence arrangement in herpes simplex virus type 1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequences. J Virol. 1976 Oct;20(1):211–221. doi: 10.1128/jvi.20.1.211-221.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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