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. 1974 Mar;13(3):590–595. doi: 10.1128/jvi.13.3.590-595.1974

Translation of mRNA from Simian Virus 40-Infected Cells into Simian Virus 40 Capsid Protein by Cell-Free Extracts

Harvey F Lodish 1,2, Robert Weinberg 1,2, Harvey L Ozer 1,2
PMCID: PMC355342  PMID: 4362863

Abstract

Messenger RNA was isolated from simian virus 40 (SV40)-infected and mock-infected cells by chromatography on poly(U) sepharose. When added to cell-free extracts from Chinese hamster ovary cells or rabbit reticulocytes, RNA from the infected cells, but not from mock-infected cells, stimulated synthesis of the major SV40 capsid protein. Identification of this species was done by sodium dodecyl sulfate gel electrophoresis, peptide mapping, and immunoprecipitation. The in vitro synthesized capsid protein was slightly different from virion assembled capsid protein, as shown by separation upon chromatography on hydroxylapatite and by minor differences in the peptide map.

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Selected References

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  1. Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson C. W., Gesteland R. F. Pattern of protein synthesis in monkey cells infected by simian virus 40. J Virol. 1972 May;9(5):758–765. doi: 10.1128/jvi.9.5.758-765.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barban S., Goor R. S. Structural proteins of simian virus 40. J Virol. 1971 Feb;7(2):198–203. doi: 10.1128/jvi.7.2.198-203.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Estes M. K., Huang E. S., Pagano J. S. Structural polypeptides of simian virus 40. J Virol. 1971 May;7(5):635–641. doi: 10.1128/jvi.7.5.635-641.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Firtel R. A., Baxter L., Lodish H. F. Actinomycin D and the regulation of enzyme biosynthesis during development of Dictyostelium discoideum. J Mol Biol. 1973 Sep 15;79(2):315–327. doi: 10.1016/0022-2836(73)90008-9. [DOI] [PubMed] [Google Scholar]
  6. Girard M., Marty L., Suarez F. Capsid proteins of Simian virus 40. Biochem Biophys Res Commun. 1970 Jul 13;40(1):97–102. doi: 10.1016/0006-291x(70)91051-x. [DOI] [PubMed] [Google Scholar]
  7. Housman D., Jacobs-Lorena M., Rajbhandary U. L., Lodish H. F. Initiation of haemoglobin synthesis by methionyl-tRNA. Nature. 1970 Aug 29;227(5261):913–918. doi: 10.1038/227913a0. [DOI] [PubMed] [Google Scholar]
  8. Jacobson M. F., Asso J., Baltimore D. Further evidence on the formation of poliovirus proteins. J Mol Biol. 1970 May 14;49(3):657–669. doi: 10.1016/0022-2836(70)90289-5. [DOI] [PubMed] [Google Scholar]
  9. Katz E., Moss B. Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin. Proc Natl Acad Sci U S A. 1970 Jul;66(3):677–684. doi: 10.1073/pnas.66.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kidwell W. R., Saral R., Martin R. G., Ozer H. L. Characterization of an endonuclease associated with simian virus 40 virions. J Virol. 1972 Sep;10(3):410–416. doi: 10.1128/jvi.10.3.410-416.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lodish H. F. Bacteriophage f2 RNA: control of translation and gene order. Nature. 1968 Oct 26;220(5165):345–350. doi: 10.1038/220345a0. [DOI] [PubMed] [Google Scholar]
  12. Lodish H. G., Housman D., Jacobsen M. Initiation of hemoglobin synthesis. Specific inhibition by antibiotics and bacteriophage ribonucleic acid. Biochemistry. 1971 Jun 8;10(12):2348–2356. doi: 10.1021/bi00788a027. [DOI] [PubMed] [Google Scholar]
  13. McDowell M. J., Joklik W. K. An in vitro protein synthesizing system from mouse L fibroblasts infected with reovirus. Virology. 1971 Sep;45(3):724–733. doi: 10.1016/0042-6822(71)90186-3. [DOI] [PubMed] [Google Scholar]
  14. McDowell M. J., Joklik W. K., Villa-Komaroff L., Lodish H. F. Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2649–2653. doi: 10.1073/pnas.69.9.2649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morrison T., Stampfer M., Baltimore D., Lodish H. F. Translation of vesicular stomatitis messenger RNA by extracts from mammalian and plant cells. J Virol. 1974 Jan;13(1):62–72. doi: 10.1128/jvi.13.1.62-72.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moss B., Rosenblum E. N. Hydroxylapatite chromatography of protein-sodium dodecyl sulfate complexes. A new method for the separation of polypeptide subunits. J Biol Chem. 1972 Aug 25;247(16):5194–5198. [PubMed] [Google Scholar]
  17. Ozer H. L. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells. J Virol. 1972 Jan;9(1):41–51. doi: 10.1128/jvi.9.1.41-51.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ozer H. L., Tegtmeyer P. Synthesis and assembly of simian virus 40. II. Synthesis of the major capsid protein and its incorporation into viral particles. J Virol. 1972 Jan;9(1):52–60. doi: 10.1128/jvi.9.1.52-60.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  20. Weber K., Kuter D. J. Reversible denaturation of enzymes by sodium dodecyl sulfate. J Biol Chem. 1971 Jul 25;246(14):4504–4509. [PubMed] [Google Scholar]
  21. Weinberg R. A., Ben-Ishai Z., Newbold J. E. Poly A associated with SV40 messenger RNA. Nat New Biol. 1972 Jul 26;238(82):111–113. doi: 10.1038/newbio238111a0. [DOI] [PubMed] [Google Scholar]
  22. Weinberg R. A., Warnaar S. O., Winocour E. Isolation and characterization of simian virus 40 ribonucleic acid. J Virol. 1972 Aug;10(2):193–201. doi: 10.1128/jvi.10.2.193-201.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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