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. 1974 May;71(5):1743–1747. doi: 10.1073/pnas.71.5.1743

Replication and Transcription of Eukaryotic DNA in Esherichia coli

John F Morrow *,†,‡, Stanley N Cohen †, Annie C Y Chang †, Herbert W Boyer §, Howard M Goodman , Robert B Helling §,ǁ
PMCID: PMC388315  PMID: 4600264

Abstract

Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.

Keywords: restriction, plasmid, transformation, recombination, ribosomal DNA

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Selected References

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