Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 May;80(10):2819–2823. doi: 10.1073/pnas.80.10.2819

H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.

R P Harvey, J A Whiting, L S Coles, P A Krieg, J R Wells
PMCID: PMC393923  PMID: 6574451

Abstract

A cDNA clone bank has been constructed from chicken embryonic RNA. Clones hybridizing poorly to embryonic histone gene probes were selected as possible variant gene transcripts. The DNA sequence of one cDNA predicts an extremely variant H2A protein (H2A.F), which is 40% divergent from the most abundant H2A protein in chicken erythrocyte chromatin. The H2A.F gene is not highly conserved across large species barriers, but in the chicken there may be a family of linked genes. The H2A.F mRNA is approximately equal to 820 base pairs in length and, unlike most other histone mRNAs, is polyadenylylated. Significantly, the H2A.F transcript shows a limited tissue distribution in the chicken embryo.

Full text

PDF
2820

Images in this article

2821Image
on p.2821
2822Image
on p.2822
2822Image
on p.2822

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appels R., Wells J. R., Williams A. F. Characterization of DNA-bound histone in the cells of the avian erythropoietic series. J Cell Sci. 1972 Jan;10(1):47–59. doi: 10.1242/jcs.10.1.47. [DOI] [PubMed] [Google Scholar]
  2. Birchmeier C., Grosschedl R., Birnstiel M. L. Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences. Cell. 1982 Apr;28(4):739–745. doi: 10.1016/0092-8674(82)90053-8. [DOI] [PubMed] [Google Scholar]
  3. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Crawford R. J., Krieg P., Harvey R. P., Hewish D. A., Wells J. R. Histone genes are clustered with a 15-kilobase repeat in the chicken genome. Nature. 1979 May 10;279(5709):132–136. doi: 10.1038/279132a0. [DOI] [PubMed] [Google Scholar]
  6. D'Andrea R., Harvey R., Wells J. R. Vertebrate histone genes: nucleotide sequence of a chicken H2A gene and regulatory flanking sequences. Nucleic Acids Res. 1981 Jul 10;9(13):3119–3128. doi: 10.1093/nar/9.13.3119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Engel J. D., Dodgson J. B. Histone genes are clustered but not tandemly repeated in the chicken genome. Proc Natl Acad Sci U S A. 1981 May;78(5):2856–2860. doi: 10.1073/pnas.78.5.2856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Engel J. D., Sugarman B. J., Dodgson J. B. A chicken histone H3 gene contains intervening sequences. Nature. 1982 Jun 3;297(5865):434–436. doi: 10.1038/297434a0. [DOI] [PubMed] [Google Scholar]
  9. Fasman G. D., Chou P. Y., Adler A. J. Prediction of the conformation of the histones. Biophys J. 1976 Oct;16(10):1201–1238. doi: 10.1016/S0006-3495(76)85768-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gould A. R., Palukaitis P., Symons R. H., Mossop D. W. Characterization of a satellite RNA associated with cucumber mosaic virus. Virology. 1978 Feb;84(2):443–455. doi: 10.1016/0042-6822(78)90261-1. [DOI] [PubMed] [Google Scholar]
  11. Graf T., Ade N., Beug H. Temperature-sensitive mutant of avian erythroblastosis virus suggests a block of differentiation as mechanism of leukaemogenesis. Nature. 1978 Oct 12;275(5680):496–501. doi: 10.1038/275496a0. [DOI] [PubMed] [Google Scholar]
  12. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Isenberg I. Histones. Annu Rev Biochem. 1979;48:159–191. doi: 10.1146/annurev.bi.48.070179.001111. [DOI] [PubMed] [Google Scholar]
  14. Krieg P. A., Robins A. J., Gait M. J., Titmas R. C., Wells J. R. Chicken histone H5: selection of a cDNA recombinant using an extended synthetic primer. Nucleic Acids Res. 1982 Mar 11;10(5):1495–1502. doi: 10.1093/nar/10.5.1495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laine B., Kmiecik D., Sautiere P., Biserte G. Primary structure of chicken erythrocyte histone H2A. Biochimie. 1978;60(2):147–150. doi: 10.1016/s0300-9084(78)80747-0. [DOI] [PubMed] [Google Scholar]
  16. Lilley D. M., Pardon J. F. Structure and function of chromatin. Annu Rev Genet. 1979;13:197–233. doi: 10.1146/annurev.ge.13.120179.001213. [DOI] [PubMed] [Google Scholar]
  17. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pantazis P., Bonner W. M. Quantitative determination of histone modification. H2A acetylation and phosphorylation. J Biol Chem. 1981 May 10;256(9):4669–4675. [PubMed] [Google Scholar]
  19. Roychoudhury R., Jay E., Wu R. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 1976 Apr;3(4):863–877. doi: 10.1093/nar/3.4.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schreier P. H., Cortese R. A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13. J Mol Biol. 1979 Mar 25;129(1):169–172. doi: 10.1016/0022-2836(79)90068-8. [DOI] [PubMed] [Google Scholar]
  22. Simpson R. T., Bergman L. W. Structure of sea urchin sperm chromatin core particle. J Biol Chem. 1980 Nov 25;255(22):10702–10709. [PubMed] [Google Scholar]
  23. Simpson R. T. Modulation of nucleosome structure by histone subtypes in sea urchin embryos. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6803–6807. doi: 10.1073/pnas.78.11.6803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  25. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Urban M. K., Franklin S. G., Zweidler A. Isolation and characterization of the histone variants in chicken erythrocytes. Biochemistry. 1979 Sep 4;18(18):3952–3960. doi: 10.1021/bi00585a017. [DOI] [PubMed] [Google Scholar]
  27. Von Holt C., Strickland W. N., Brandt W. F., Strickland M. S. More histone structures. FEBS Lett. 1979 Apr 15;100(2):201–218. doi: 10.1016/0014-5793(79)80337-3. [DOI] [PubMed] [Google Scholar]
  28. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. West M. H., Bonner W. M. Histone 2A, a heteromorphous family of eight protein species. Biochemistry. 1980 Jul 8;19(14):3238–3245. doi: 10.1021/bi00555a022. [DOI] [PubMed] [Google Scholar]
  30. Wu R. S., Bonner W. M. Separation of basal histone synthesis from S-phase histone synthesis in dividing cells. Cell. 1981 Dec;27(2 Pt 1):321–330. doi: 10.1016/0092-8674(81)90415-3. [DOI] [PubMed] [Google Scholar]
  31. Wu R. S., Nishioka D., Bonner W. M. Differential conservation of histone 2A variants between mammals and sea urchins. J Cell Biol. 1982 May;93(2):426–431. doi: 10.1083/jcb.93.2.426. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES