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. 1972 Mar;69(3):681–685. doi: 10.1073/pnas.69.3.681

Characterization of Messenger Ribonucleoprotein and Messenger RNA from KB Cells

Ajit Kumar 1,*, Uno Lindberg 1
PMCID: PMC426534  PMID: 4501582

Abstract

Messenger ribonucleoprotein and mRNA from KB-cells were isolated under conditions designed to minimize nonspecific RNA-protein interaction and to minimize degradation by contaminating ribonucleases. A large fraction, 60-70%, of the messenger ribonucleoprotein from polysomes dissociated in vitro by either EDTA or puromycin sedimented faster than the large ribosome subunit. Messenger ribonucleoprotein particles with sedimentation coefficients up to 200 S were observed. Released mRNA was also large, with maximal molecular weights around 5 × 106.

Keywords: eukaryotic cells, polysomes, molecular weight, density gradient centrifugation

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Selected References

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