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. 1976 Jul;73(7):2351–2355. doi: 10.1073/pnas.73.7.2351

Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function.

G N Bennett, M E Schweingruber, K D Brown, C Squires, C Yanofsky
PMCID: PMC430561  PMID: 1088825

Abstract

The nucleotide sequence of the region preceding the transcription initiation site of the tryptophan operon of Escherichia coli was determined. Essentially all of the trp operator precedes the transcribed portion of the operon. The deduced sequence contains the recognition site of endonuclease Hpa I. This site is protected from Hpa I cleavage by RNA polymerase and by trp repressor. Regions of 2-fold symmetry are present in the DNA sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bronson M. J., Squires C., Yanofsky C. Nucleotide sequences from tryptophan messenger RNA of Escherichia coli: the sequence corresponding to the amino-terminal region of the first polypeptide specified by the operon. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2335–2339. doi: 10.1073/pnas.70.8.2335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dugaiczyk A., Boyer H. W., Goodman H. M. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975 Jul 25;96(1):171–184. doi: 10.1016/0022-2836(75)90189-8. [DOI] [PubMed] [Google Scholar]
  3. Dykes G., Bambara R., Marians K., Wu R. On the statistical significance of primary structural features found in DNA-protein interaction sites. Nucleic Acids Res. 1975 Mar;2(3):327–345. doi: 10.1093/nar/2.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garfin D. E., Goodman H. M. Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae. Biochem Biophys Res Commun. 1974 Jul 10;59(1):108–116. doi: 10.1016/s0006-291x(74)80181-6. [DOI] [PubMed] [Google Scholar]
  5. Gilbert W., Maxam A. The nucleotide sequence of the lac operator. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581–3584. doi: 10.1073/pnas.70.12.3581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Imamoto F., Tani S. Diversity of regulation of genetic transcription. Nat New Biol. 1972 Dec 6;240(101):172–175. doi: 10.1038/newbio240172a0. [DOI] [PubMed] [Google Scholar]
  7. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maniatis T., Ptashne M., Backman K., Kield D., Flashman S., Jeffrey A., Maurer R. Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda. Cell. 1975 Jun;5(2):109–113. doi: 10.1016/0092-8674(75)90018-5. [DOI] [PubMed] [Google Scholar]
  9. Maniatis T., Ptashne M., Barrell B. G., Donelson J. Sequence of a repressor-binding site in the DNA of bacteriophage lamda. Nature. 1974 Aug 2;250(465):394–397. doi: 10.1038/250394a0. [DOI] [PubMed] [Google Scholar]
  10. Pirrotta V. Sequence of the OR operator of phage lambda. Nature. 1975 Mar 13;254(5496):114–117. doi: 10.1038/254114a0. [DOI] [PubMed] [Google Scholar]
  11. Platt T., Yanofsky C. An intercistronic region and ribosome-binding site in bacterial messenger RNA. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2399–2403. doi: 10.1073/pnas.72.6.2399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pribnow D. Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites. J Mol Biol. 1975 Dec 15;99(3):419–443. doi: 10.1016/s0022-2836(75)80136-7. [DOI] [PubMed] [Google Scholar]
  13. Pribnow D. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci U S A. 1975 Mar;72(3):784–788. doi: 10.1073/pnas.72.3.784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rose J. K., Squires C. L., Yanofsky C., Yang H. L., Zubay G. Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan. Nat New Biol. 1973 Oct 3;245(144):133–137. doi: 10.1038/newbio245133a0. [DOI] [PubMed] [Google Scholar]
  15. Schaller H., Gray C., Herrmann K. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. Proc Natl Acad Sci U S A. 1975 Feb;72(2):737–741. doi: 10.1073/pnas.72.2.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sekiua T., Ormondt H. V., Khorana H. G. The nucleotide sequence in the promoter region of the fene for an Escherichia coli tyrosine transfer ribonucleic acid. J Biol Chem. 1975 Feb 10;250(3):1087–1098. [PubMed] [Google Scholar]
  17. Squires C. L., Lee F. D., Yanofsky C. Interaction of the trp repressor and RNA polymerase with the trp operon. J Mol Biol. 1975 Feb 15;92(1):93–111. doi: 10.1016/0022-2836(75)90093-5. [DOI] [PubMed] [Google Scholar]
  18. Walz A., Pirrotta V. Sequence of the PR promoter of phage lambda. Nature. 1975 Mar 13;254(5496):118–121. doi: 10.1038/254118a0. [DOI] [PubMed] [Google Scholar]
  19. Yanofsky C., Horn V., Bonner M., Stasiowski S. Polarity and enzyme functions in mutants of the first three genes of the tryptophan operon of Escherichia coli. Genetics. 1971 Dec;69(4):409–433. doi: 10.1093/genetics/69.4.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zalkin H., Yanofsky C., Squires C. L. Regulated in vitro synthesis of Escherichia coli tryptophan operon messenger ribonucleic acid and enzymes. J Biol Chem. 1974 Jan 25;249(2):465–475. [PubMed] [Google Scholar]

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