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. 1975 Apr;72(4):1427–1430. doi: 10.1073/pnas.72.4.1427

Variants of a mouse myeloma cell line that synthesize immunoglobulin heavy chains having an altered serotype.

J L Preud'Homme, B K Birshtein, M D Scharff
PMCID: PMC432548  PMID: 805428

Abstract

Cells of the MPC-11 mouse myeloma cell line, which produces an IgG2b immunoglobulin, were subjected to mutagenesis with Melphalan or with ICR-191, after which they were cloned in soft agar. Approximately 0.5 percent of the clones produced altered heavy chains that: (i) were the same size as or larger than the parent; (ii) no longer recognized by antibody specific for the parent gamma2b subclass; (iv) were recognized by antibody against the Fab (NH2-terminal half) of the parental immunoglobulin, but lacked some of the antigenic determinants of the Fc (COOH-terminal half) of the heavy chain; (v) lacked many of the tryptic/chymotryptic peptides found in the parent; (vi) contained tryptic/chymotryptic peptides that were not present in the parent but were present in an unrelated gamma2a myeloma heavy chain; and (vii) assembled with light chains by a pathway typical of IgG2a myelomas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumal R., Birshtein B. K., Coffino P., Scharff M. D. Mutations in immunoglobulin-producing mouse myeloma cells. Science. 1973 Oct 12;182(4108):164–166. doi: 10.1126/science.182.4108.164. [DOI] [PubMed] [Google Scholar]
  2. Baumal R., Potter M., Scharff M. D. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. 3. Assembly of the three subclasses of IgG. J Exp Med. 1971 Nov 1;134(5):1316–1334. doi: 10.1084/jem.134.5.1316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Birshtein B. K., Preud'homme J. L., Scharff M. D. Variants of mouse myeloma cells that produce short immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3478–3482. doi: 10.1073/pnas.71.9.3478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coffino P., Baumal R., Laskov R., Scharff M. D. Cloning of mouse myeloma cells and detection of rare variants. J Cell Physiol. 1972 Jun;79(3):429–440. doi: 10.1002/jcp.1040790313. [DOI] [PubMed] [Google Scholar]
  5. Coffino P., Scharff M. D. Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc Natl Acad Sci U S A. 1971 Jan;68(1):219–223. doi: 10.1073/pnas.68.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gally J. A., Edelman G. M. Somatic translocation of antibody genes. Nature. 1970 Jul 25;227(5256):341–348. doi: 10.1038/227341a0. [DOI] [PubMed] [Google Scholar]
  7. Kuehl W. M., Scharff M. D. Synthesis of a carboxyl-terminal (constant region) fragment of the immunoglobulin light chain by a mouse myeloma cell line. J Mol Biol. 1974 Nov 5;89(3):409–421. doi: 10.1016/0022-2836(74)90472-0. [DOI] [PubMed] [Google Scholar]
  8. Kunkel H. G., Natvig J. B., Joslin F. G. A "Lepore" type of hybrid gamma-globulin. Proc Natl Acad Sci U S A. 1969 Jan;62(1):144–149. doi: 10.1073/pnas.62.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laskov R., Scharff M. D. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J Exp Med. 1970 Mar 1;131(3):515–541. doi: 10.1084/jem.131.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morrison S. L., Baumal R., Birshtein B. K., Kuehl U. M., Preud'homme J. L., Frank L., Jasek T., Scharff M. D. The identification of mouse myeloma cells which have undergone mutations in immunoglobulin production. Soc Gen Physiol Ser. 1974;29:233–244. [PubMed] [Google Scholar]
  11. Natvig J. B., Kunkel H. G. A HYBRID IgG4-IgG2 immunoglobulin. J Immunol. 1974 Apr;112(4):1277–1284. [PubMed] [Google Scholar]
  12. Prend'homme J. L., Buxbaum J., Scharff M. D. Mutagenesis of mouse myeloma cells with 'Melphalan'. Nature. 1973 Oct 12;245(5424):320–322. doi: 10.1038/245320a0. [DOI] [PubMed] [Google Scholar]
  13. Warner N. L., Herzenberg L. A., Goldstein G. Immunoglobulin isoantigens (allotypes) in the mouse. II. Allotypic analysis of three gammaG2-myeloma proteins from (NZB x BALB/c)F1 hybrids and of normal gammaG2-globulins. J Exp Med. 1966 Apr 1;123(4):707–721. doi: 10.1084/jem.123.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]

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