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. 1975 Sep;72(9):3661–3665. doi: 10.1073/pnas.72.9.3661

Absence of H-2 antigens capable of reacting with cytotoxic T cells on a teratoma line expressing a T/t locus antigen.

J Forman, E S Vitetta
PMCID: PMC433056  PMID: 52873

Abstract

An established cell line of murine teratoma cells (F9), which lacks serologically detectable H-2 that is determined by a wild-type T/t locus gene. These cells are not killed and do not react with cytotoxic T cells sensitized to H-2 antigens in a cell-mediated lympholysis assay. Modification of spleen cells from the strain or origin (129) of this teratoma line with trinitrobenzene sulfonic acid allows the generation of syngeneic killer cells that display a cytotoxic effect against trinitrophenyl-modified splenic targets, but not against trinitrophenyl-modified F9 targets. Thus, the F9 antigen is structurally similar to H-2b but does not act as a target antigen in the cell-mediated lympholysis assay for anti-H-2b cytotoxic T cells, nor does it crossreact with H-2b antigens at the T cell level.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alter B. J., Schendel D. J., Bach M. L., Bach F. H., Klein J., Stimpfling J. H. Cell-mediated lympholysis. Importance of serologically defined H-2 regions. J Exp Med. 1973 May 1;137(5):1303–1309. doi: 10.1084/jem.137.5.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Artzt K., Dubois P., Bennett D., Condamine H., Babinet C., Jacob F. Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988–2992. doi: 10.1073/pnas.70.10.2988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Artzt K., Jacob F. Letter: Absence of serologically detectable H-2 on primitive teratocarcinoma cells in culture. Transplantation. 1974 Jun;17(6):632–634. doi: 10.1097/00007890-197406000-00015. [DOI] [PubMed] [Google Scholar]
  4. BENNETT D. ABNORMALITIES ASSOCIATED WITH A CHROMOSOME REGION IN THE MOUSE. II. EMBRYOLOGICAL EFFECTS OF LETHAL ALLELES IN THE T-REGION. Science. 1964 Apr 17;144(3616):263–267. [PubMed] [Google Scholar]
  5. Bonavida B. Studies on the induction and expression of T cell-mediated immunity. II. Antiserum blocking of cell-mediated cytolysis. J Immunol. 1974 Apr;112(4):1308–1321. [PubMed] [Google Scholar]
  6. Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
  7. Forman J., Möller G. Generation of cytotoxic lymphocytes in mixed lymphocyte reactions. I. Specificity of the effector cells. J Exp Med. 1973 Sep 1;138(3):672–685. doi: 10.1084/jem.138.3.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Forman J. On the role of the H-2 histocompatibility complex in determining the specificity of cytotoxic effector cells sensitized against syngeneic trinitrophenyl-modified targets. J Exp Med. 1975 Aug 1;142(2):403–418. doi: 10.1084/jem.142.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gooding L. R., Edidin M. Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H-2 antigens on tumor cells. J Exp Med. 1974 Jul 1;140(1):61–78. doi: 10.1084/jem.140.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Häyry P., Andersson L. C., Nordling S., Virolainen M. Allograft response in vitro. Transplant Rev. 1972;12:91–140. doi: 10.1111/j.1600-065x.1972.tb00054.x. [DOI] [PubMed] [Google Scholar]
  11. Kahan B. W., Ephrussi B. Developmental potentialities of clonal in vitro cultures of mouse testicular teratoma. J Natl Cancer Inst. 1970 May;44(5):1015–1036. [PubMed] [Google Scholar]
  12. Katz D. H., Graves M., Dorf M. E., Dimuzio H., Benacerraf B. Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex. J Exp Med. 1975 Jan 1;141(1):263–268. doi: 10.1084/jem.141.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lesley J., Hyman R., Dennert G. Effect of antigen density on complement-mediated lysis, T-cell-mediated killing, and antigenic modulation. J Natl Cancer Inst. 1974 Dec;53(6):1759–1765. [PubMed] [Google Scholar]
  14. MOLLER E., MOLLER G. Quantitative studies of the sensitivity of normal and neoplastic mouse cells to the cytotoxic action of isoantibodies. J Exp Med. 1962 Mar 1;115:527–553. doi: 10.1084/jem.115.3.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nabholz M., Vives J., Young H. M., Meo T., Miggiano V., Rijnbeek A., Shreffler D. C. Cell-mediated cell lysis in vitro: genetic control of killer cell production and target specificities in the mouse. Eur J Immunol. 1974 May;4(5):378–387. doi: 10.1002/eji.1830040514. [DOI] [PubMed] [Google Scholar]
  16. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosenthal M. D., Wishnow R. M., Sato G. H. In vitro growth and differetiation of clonal populations of multipotential mouse clls derived from a transplantable testicular teratocarcinoma. J Natl Cancer Inst. 1970 May;44(5):1001–1014. [PubMed] [Google Scholar]
  18. STEVENS L. C. Studies on transplantable testicular teratomas of strain 129 mice. J Natl Cancer Inst. 1958 Jun;20(6):1257–1275. doi: 10.1093/jnci/20.6.1257. [DOI] [PubMed] [Google Scholar]
  19. Shearer G. M. Cell-mediated cytotoxicity to trinitrophenyl-modified syngeneic lymphocytes. Eur J Immunol. 1974 Aug;4(8):527–533. doi: 10.1002/eji.1830040802. [DOI] [PubMed] [Google Scholar]
  20. Shearer G. M., Rehn T. G., Garbarino C. A. Cell-mediated lympholysis of trinitrophenyl-modified autologous lymphocytes. Effector cell specificity to modified cell surface components controlled by H-2K and H-2D serological regions of the murine major histocompatibility complex. J Exp Med. 1975 Jun 1;141(6):1348–1364. doi: 10.1084/jem.141.6.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Snell G. D., Graff R. J., Cherry M. Histocompatibility genes of mice. XI. Evidence establishing a new histocompatibility locus, H-12, and new H-2 allele, H-2bc. Transplantation. 1971 Jun;11(6):525–530. [PubMed] [Google Scholar]
  22. Vitetta E. S., Artzt K., Bennett D., Boyse E. A., Jacob F. Structural similarities between a product of the T/t-locus isolated from sperm and teratoma cells, and H-2 antigens isolated from splenocytes. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3215–3219. doi: 10.1073/pnas.72.8.3215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wunderlich J. R., Canty T. G. Cell mediated immunity induced in vitro. Nature. 1970 Oct 3;228(5266):62–63. doi: 10.1038/228062a0. [DOI] [PubMed] [Google Scholar]

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