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. 1974 Sep;71(9):3465–3469. doi: 10.1073/pnas.71.9.3465

A New Relaxed Mutant of Escherichia coli with an Altered 50S Ribosomal Subunit

J D Friesen *, N P Fiil †, J M Parker *, William A Haseltine ‡
PMCID: PMC433794  PMID: 4610577

Abstract

A new relaxed mutant called rel C has been isolated from a rel A+/rel A+ partial diploid strain. The rel C mutant is unable to synthesize ppGpp or pppGpp in vivo in response to amino acid starvation or in vitro, but can synthesize these nucleotides in a shift-down. Rel C maps near rif. Studies in vitro demonstrate the lesion to be probably in one of the 50S ribosomal proteins that can be removed by 1.0 M LiCl.

Keywords: RNA regulation, guanosine tetraphosphate, in vitro synthesis of ppGpp and pppGpp, genetic mapping

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Selected References

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