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. 1982;1(3):345–349. doi: 10.1002/j.1460-2075.1982.tb01172.x

Control of HLA-A,B,C synthesis and expression in interferon-treated cells.

O R Burrone, C Milstein
PMCID: PMC553047  PMID: 6765173

Abstract

Cytofluorimetric analyses with monoclonal antibodies were used to follow the specific effect of highly purified human leucocyte interferon (IFN-alpha) on the expression of transplantation antigens (HLA)1 in Molt 4, a thymus leukaemia cell line. After a lag period of approximately 10 h the amount of HLA steadily increases to reach a maximum at day six of approximately 10 times its original. The effect is reversible and due to an increase in the rate of synthesis of HLA and beta 2m molecules as demonstrated by analysis of [35S]methionine incorporation into specific proteins after a 3-h pulse of Molt 4 cells treated with IFN-alpha for one or six days. A dramatic increase in the amount of mRNA that hybridised with a [32P]cDNA probe containing HLA sequences has also been demonstrated. Surface iodination of IFN-treated Molt 4 cells showed an increase in the iodinated HLA and beta 2m chains and in addition another, apparently unrelated, component with an apparent mol. wt. of 16 000. This component, which appears after incubations with IFN-alpha for longer than 1 h at 37 degrees C, was also detected in the B lymphoid cell lines RAJI and DAUDI.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams A., Strander H., Cantell K. Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. J Gen Virol. 1975 Aug;28(2):207–217. doi: 10.1099/0022-1317-28-2-207. [DOI] [PubMed] [Google Scholar]
  2. Barnstable C. J., Bodmer W. F., Brown G., Galfre G., Milstein C., Williams A. F., Ziegler A. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978 May;14(1):9–20. doi: 10.1016/0092-8674(78)90296-9. [DOI] [PubMed] [Google Scholar]
  3. Bradstock K. F., Janossy G., Bollum F. J., Milstein C. Anomalous phenotype in thymic acute lymphoblastic leukaemia. Nature. 1980 Apr 3;284(5755):455–457. doi: 10.1038/284455a0. [DOI] [PubMed] [Google Scholar]
  4. Farrell P. J., Broeze R. J., Lengyel P. Accumulation of an mRNA and protein in interferon-treated Ehrlich ascites tumour cells. Nature. 1979 Jun 7;279(5713):523–525. doi: 10.1038/279523a0. [DOI] [PubMed] [Google Scholar]
  5. Fellous M., Bono R., Hyafil F., Gresser I. Interferon enhances the amount of membrane-bound beta2-microglobulin and its release from human Burkitt cells. Eur J Immunol. 1981 Jun;11(6):524–526. doi: 10.1002/eji.1830110616. [DOI] [PubMed] [Google Scholar]
  6. Fellous M., Kamoun M., Gresser I., Bono R. Enhanced expression of HLA antigens and beta 2-microglobulin on interferon-treated human lymphoid cells. Eur J Immunol. 1979 Jun;9(6):446–449. doi: 10.1002/eji.1830090606. [DOI] [PubMed] [Google Scholar]
  7. Goodfellow P. N., Jones E. A., Van Heyningen V., Solomon E., Bobrow M., Miggiano V., Bodmer W. F. The beta2-microglobulin gene is on chromosome 15 and not in the HL-A region. Nature. 1975 Mar 20;254(5497):267–269. doi: 10.1038/254267a0. [DOI] [PubMed] [Google Scholar]
  8. Gupta S. L., Rubin B. Y., Holmes S. L. Interferon action: induction of specific proteins in mouse and human cells by homologous interferons. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4817–4821. doi: 10.1073/pnas.76.10.4817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heron I., Hokland M., Berg K. Enhanced expression of beta2-microglobulin and HLA antigens on human lymphoid cells by interferon. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6215–6219. doi: 10.1073/pnas.75.12.6215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hilfenhaus J., Damm H., Karges H. E., Manthey K. F. Growth inhibition of human lymphoblastoid Daudi cells in vitro by interferon preparations. Arch Virol. 1976;51(1-2):87–97. doi: 10.1007/BF01317837. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Laskey R. A., Mills A. D. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977 Oct 15;82(2):314–316. doi: 10.1016/0014-5793(77)80609-1. [DOI] [PubMed] [Google Scholar]
  13. Lindahl P., Gresser I., Leary P., Tovey M. Interferon treatment of mice: enhanced expression of histocompatibility antigens on lymphoid cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1284–1287. doi: 10.1073/pnas.73.4.1284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McMichael A. J., Pilch J. R., Galfré G., Mason D. Y., Fabre J. W., Milstein C. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol. 1979 Mar;9(3):205–210. doi: 10.1002/eji.1830090307. [DOI] [PubMed] [Google Scholar]
  15. Secher D. S., Burke D. C. A monoclonal antibody for large-scale purification of human leukocyte interferon. Nature. 1980 Jun 12;285(5765):446–450. doi: 10.1038/285446a0. [DOI] [PubMed] [Google Scholar]
  16. Sood A. K., Pereira D., Weissman S. M. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc Natl Acad Sci U S A. 1981 Jan;78(1):616–620. doi: 10.1073/pnas.78.1.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stewart W. E., 2nd, Gresser I., Tovey M. G., Bandu M., Le Goff S. Identification of the cell multiplication inhibitory factors in interferon preparations as interferons. Nature. 1976 Jul 22;262(5566):300–302. doi: 10.1038/262300a0. [DOI] [PubMed] [Google Scholar]
  18. Tada N., Tanigaki N., Pressman D. Human cell membrane components bound to beta2-microglobulin in T cell-type cell lines. J Immunol. 1978 Feb;120(2):513–519. [PubMed] [Google Scholar]
  19. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vignaux F., Gresser I. Differential effects of interferon on the expression of H-2K, H-2D, and Ia antigens on mouse lymphocytes. J Immunol. 1977 Feb;118(2):721–723. [PubMed] [Google Scholar]
  21. Ziegler A., Milstein C. A small polypeptide different from beta2-microglobin associated with a human cell surface antigen. Nature. 1979 May 17;279(5710):243–244. doi: 10.1038/279243a0. [DOI] [PubMed] [Google Scholar]

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