Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1983;2(1):121–124. doi: 10.1002/j.1460-2075.1983.tb01392.x

Polymorphism and complexity of the human DC and murine I-A alpha chain genes.

C Auffray 1, A Ben-Nun 1, M Roux-Dosseto 1, R N Germain 1, J G Seidman 1, J L Strominger 1
PMCID: PMC555098  PMID: 11894898

Abstract

A cDNA clone encoding the human B cell alloantigen DC alpha chain (pDCH1) has been used to analyse the structure of the human and murine major histocompatibility complexes by the DNA filter hybridization technique. The pDCH1 probe hybridizes to a single DNA sequence present on chromosome 17 in the mouse genome. A restriction enzyme polymorphism enables us to map this sequence to the I-A subregion. Extensive restriction enzyme polymorphism detected in HLA-DR homozygous typing cells is reminiscent of the DR/MT linkage disequilibrium groups, suggesting that the pDCH1 probe could be useful for haplotype typing in the human population. The HLA-DR region appears more complex than the I region since a second DC-like hybridizing sequence is detected in the human genome in these experiments.

Full text

PDF
124

Images in this article

122Fig. 1.
on p.122
122Fig. 2.
on p.122
123Fig. 3.
on p.123

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auffray C., Korman A. J., Roux-Dosseto M., Bono R., Strominger J. L. cDNA clone for the heavy chain of the human B cell alloantigen DC1: strong sequence homology to the HLA-DR heavy chain. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6337–6341. doi: 10.1073/pnas.79.20.6337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benacerraf B. Role of MHC gene products in immune regulation. Science. 1981 Jun 12;212(4500):1229–1238. doi: 10.1126/science.6165083. [DOI] [PubMed] [Google Scholar]
  3. Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cami B., Brégégère F., Abastado J. P., Kourilsky P. Multiple sequences related to classical histocompatibility antigens in the mouse genome. Nature. 1981 Jun 25;291(5817):673–675. doi: 10.1038/291673a0. [DOI] [PubMed] [Google Scholar]
  5. Coligan J. E., Kindt T. J., Uehara H., Martinko J., Nathenson S. G. Primary structure of a murine transplantation antigen. Nature. 1981 May 7;291(5810):35–39. doi: 10.1038/291035a0. [DOI] [PubMed] [Google Scholar]
  6. Cook R. G., Vitetta E. S., Uhr J. W., Capra J. D. Structural studies on the murine Ia alloantigens--III. Tryptic peptide comparisons of allelic products of the I-E/C sub-region. Mol Immunol. 1979 Jan;16(1):29–35. doi: 10.1016/0161-5890(79)90024-5. [DOI] [PubMed] [Google Scholar]
  7. De Kretser T. A., Crumpton M. J., Bodmer J. G., Bodmer W. F. Two-dimensional gel analysis of the polypeptides precipitated by a polymorphic HLA-DR1,2,w6 monoclonal antibody: evidence for a third locus. Eur J Immunol. 1982 Jul;12(7):600–606. doi: 10.1002/eji.1830120713. [DOI] [PubMed] [Google Scholar]
  8. Evans G. A., Margulies D. H., Camerini-Otero R. D., Ozato K., Seidman J. G. Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1994–1998. doi: 10.1073/pnas.79.6.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goyert S. M., Shively J. E., Silver J. Biochemical characterization of a second family of human Ia molecules, HLA-DS, equivalent to murine I-A subregion molecules. J Exp Med. 1982 Aug 1;156(2):550–566. doi: 10.1084/jem.156.2.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heidmann O., Rougeon F. Multiple sequences related to a constant-region kappa light chain gene in the rabbit genome. Cell. 1982 Mar;28(3):507–513. doi: 10.1016/0092-8674(82)90205-7. [DOI] [PubMed] [Google Scholar]
  11. Karr R. W., Kannapell C. C., Stein J. A., Gebel H. M., Mann D. L., Duquesnoy R. J., Fuller T. C., Rodey G. E., Schwartz B. D. Molecular relationships of the human B cell alloantigens, MT2, MB3, MT4, and DR5. J Immunol. 1982 Apr;128(4):1809–1818. [PubMed] [Google Scholar]
  12. Klein J. The major histocompatibility complex of the mouse. Science. 1979 Feb 9;203(4380):516–521. doi: 10.1126/science.104386. [DOI] [PubMed] [Google Scholar]
  13. Korman A. J., Knudsen P. J., Kaufman J. F., Strominger J. L. cDNA clones for the heavy chain of HLA-DR antigens obtained after immunopurification of polysomes by monoclonal antibody. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1844–1848. doi: 10.1073/pnas.79.6.1844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Larhammar D., Gustafsson K., Claesson L., Bill P., Wiman K., Schenning L., Sundelin J., Widmark E., Peterson P. A., Rask L. Alpha chain of HLA-DR transplantation antigens is a member of the same protein superfamily as the immunoglobulins. Cell. 1982 Aug;30(1):153–161. doi: 10.1016/0092-8674(82)90021-6. [DOI] [PubMed] [Google Scholar]
  15. Lee J. S., Trowsdale J., Bodmer W. F. cDNA clones coding for the heavy chain of human HLA-DR antigen. Proc Natl Acad Sci U S A. 1982 Jan;79(2):545–549. doi: 10.1073/pnas.79.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Long E. O., Wake C. T., Strubin M., Gross N., Accolla R. S., Carrel S., Mach B. Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7465–7469. doi: 10.1073/pnas.79.23.7465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Margulies D. H., Evans G. A., Flaherty L., Seidman J. G. H-2-like genes in the Tla region of mouse chromosome 17. Nature. 1982 Jan 14;295(5845):168–170. doi: 10.1038/295168a0. [DOI] [PubMed] [Google Scholar]
  18. Moutsopoulos H. M., Mann D. L., Johnson A. H., Chused T. M. Genetic differences between primary and secondary sicca syndrome. N Engl J Med. 1979 Oct 4;301(14):761–763. doi: 10.1056/NEJM197910043011405. [DOI] [PubMed] [Google Scholar]
  19. Reinertsen J. L., Klippel J. H., Johnson A. H., Steinberg A. D., Decker J. L., Mann D. L. B-lymphocyte alloantigens associated with systemic lupus erythematosus. N Engl J Med. 1978 Sep 7;299(10):515–518. doi: 10.1056/NEJM197809072991004. [DOI] [PubMed] [Google Scholar]
  20. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  21. Shackelford D. A., Kaufman J. F., Korman A. J., Strominger J. L. HLA-DR antigens: structure, separation of subpopulations, gene cloning and function. Immunol Rev. 1982;66:133–187. doi: 10.1111/j.1600-065x.1982.tb00437.x. [DOI] [PubMed] [Google Scholar]
  22. Shackelford D. A., Mann D. L., van Rood J. J., Ferrara G. B., Strominger J. L. Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigen. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4566–4570. doi: 10.1073/pnas.78.7.4566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smiley J. R., Steege D. A., Juricek D. K., Summers W. P., Ruddle F. H. A herpes simplex virus 1 integration site in the mouse genome defined by somatic cell genetic analysis. Cell. 1978 Oct;15(2):455–468. doi: 10.1016/0092-8674(78)90015-6. [DOI] [PubMed] [Google Scholar]
  24. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  25. Steinmetz M., Frelinger J. G., Fisher D., Hunkapiller T., Pereira D., Weissman S. M., Uehara H., Nathenson S., Hood L. Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981 Apr;24(1):125–134. doi: 10.1016/0092-8674(81)90508-0. [DOI] [PubMed] [Google Scholar]
  26. Tosi R., Tanigaki N., Centis D., Ferrara G. B., Pressman D. Immunological dissection of human Ia molecules. J Exp Med. 1978 Dec 1;148(6):1592–1611. doi: 10.1084/jem.148.6.1592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Trägärdh L., Rask L., Wiman K., Fohlman J., Peterson P. A. Complete amino acid sequence of pooled papain-solubilized HLA-A, -B, and -C antigens: relatedness to immunoglobulins and internal homologies. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1129–1133. doi: 10.1073/pnas.77.2.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wiman K., Larhammar D., Claesson L., Gustafsson K., Schenning L., Bill P., Böhme J., Denaro M., Dobberstein B., Hammerling U. Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1703–1707. doi: 10.1073/pnas.79.6.1703. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES