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. 1980 Oct;77(10):6081–6085. doi: 10.1073/pnas.77.10.6081

Molecular cloning of a human histocompatibility antigen cDNA fragment.

H L Ploegh, H T Orr, J L Strominger
PMCID: PMC350217  PMID: 6934534

Abstract

A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was established by its ability to hybridize to HLA heavy chain mRNA and by nucleotide sequence analysis. The pHLA-1 cDNA insert (approximately 525 base pairs) corresponds to the COOH-terminal 46 amino acids of an HLA-A, -B, or -C antigen (15 residues from the hydrophobic region and the remainder from the COOH-termial hydrophilic region), together with a portion of the 3' untranslated region of the mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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